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Literature summary extracted from

  • Thoden, J.B.; Kim, J.; Raushel, F.M.; Holden, H.M.
    The catalytic mechanism of galactose mutarotase (2003), Protein Sci., 12, 1051-1059.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.3.3 mutants H96N, H170N, D243N, D243A, E304Q, E304A, complexed with either D-glucose or D-galactose Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.3 D243A drastic decrease in activity Lactococcus lactis
5.1.3.3 D243N decrease in kcat-value, increase in Km-value Lactococcus lactis
5.1.3.3 E304A no enzymic activity Lactococcus lactis
5.1.3.3 E304Q no enzymic activity Lactococcus lactis
5.1.3.3 H170N no enzymic activity Lactococcus lactis
5.1.3.3 H96N large decrease in kcat-value Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.3 19
-
 alpha-L-arabinose mutant D243A, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 20
-
 alpha-D-galactose wild-type, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 21
-
 alpha-D-xylose wild-type, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 32
-
 alpha-L-arabinose wild-type, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 33
-
 alpha-D-xylose mutant D243N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 34
-
 alpha-D-glucose wild-type, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 38
-
 alpha-D-galactose mutant D243N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 46
-
 alpha-D-glucose mutant H96N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 52
-
 alpha-D-galactose mutant D243A, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 58
-
 alpha-L-arabinose mutant H96N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 86
-
 alpha-D-xylose mutant H96N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 120
-
 alpha-D-glucose mutant D243N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 170
-
 alpha-L-arabinose mutant D243N, pH 7.6, 25°C Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.3 Lactococcus lactis
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.1.3.3 alpha-D-glucose = beta-D-glucose residues E304 and H170 are critical for catalysis and H96 and D243 are important for substrate positioning Lactococcus lactis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.3 alpha-D-galactose
-
 Lactococcus lactis beta-D-galactose
-
 ?
5.1.3.3 alpha-D-glucose
-
 Lactococcus lactis beta-D-glucose
-
 ?
5.1.3.3 alpha-D-xylose
-
 Lactococcus lactis beta-D-xylose
-
 ?
5.1.3.3 alpha-L-arabinose
-
 Lactococcus lactis beta-L-arabinose
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.3 1
-
 alpha-D-xylose mutant D243A, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 2.1
-
 alpha-D-glucose mutant D243A, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 9
-
 alpha-L-arabinose mutant D243A, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 17
-
 alpha-D-glucose mutant H96N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 50
-
 alpha-D-galactose mutant D243A, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 62
-
 alpha-L-arabinose mutant H96N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 92
-
 alpha-D-xylose mutant H96N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 140
-
 alpha-D-xylose mutant D243N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 170
-
 alpha-D-galactose mutant H96N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 250
-
 alpha-D-glucose mutant D243N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 410
-
 alpha-D-xylose wild-type, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 430
-
 alpha-D-glucose wild-type, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 1500
-
 alpha-L-arabinose mutant D243N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 2400
-
 alpha-D-galactose mutant D243N, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 3700
-
 alpha-D-galactose wild-type, pH 7.6, 25°C Lactococcus lactis
5.1.3.3 5500
-
 alpha-L-arabinose wild-type, pH 7.6, 25°C Lactococcus lactis