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Literature summary extracted from

  • Tsuge, H.; Sakuraba, H.; Kobe, T.; Kujime, A.; Katunuma, N.; Ohshima, T.
    Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-A resolution: a large conformational change in ADP-dependent glucokinase (2002), Protein Sci., 11, 2456-2463.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.147 gene PH0589, DNA and amino acid sequence determination and analysis, expression of the N-terminally His-tagged in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.147 purified recombinant N-terminally His-tagged apoenzyme, hanging drop vapour diffusion method, 20°C, 10 mg/ml protein in solution is mixed with an equal volume of 0.003 ml of mother liquor containing 9-13% PEG 6000, 0.2 M Li2SO4, and 0.1 M citrate buffer, pH 3.6, heavy atom derivatization with HgCl2, X-ray diffraction structure determination and analysis at 2.0 A resolution, single isomorphous replacement with an anomalous scattering Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.147 Mg2+
-
 Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.147 ADP + D-glucose Pyrococcus horikoshii
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Pyrococcus horikoshii OT-3
-
 AMP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.147 Pyrococcus horikoshii
-
 strain OT3, gene PH0589
-
2.7.1.147 Pyrococcus horikoshii OT-3
-
 strain OT3, gene PH0589
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.147 recombinant N-terminally His-tagged enzyme from Escherichia coli by heat treatment and nickel affinity chromatography Pyrococcus horikoshii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.147 additional information
-
 activity determination in a coupled assay with glucose 6-phosphate dehydrogenase and NADP+ Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.147 ADP + D-glucose
-
 Pyrococcus horikoshii AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Pyrococcus horikoshii OT-3 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 additional information substrate-induced fit, binding of ADP induces large conformational changes Pyrococcus horikoshii ?
-
 ?
2.7.1.147 additional information substrate-induced fit, binding of ADP induces large conformational changes Pyrococcus horikoshii OT-3 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.147 ADP-dependent glucokinase
-
 Pyrococcus horikoshii
2.7.1.147 ADPGK
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.147 50
-
 assay at Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.147 7.5
-
 assay at Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.147 ADP binding site structure composed of 2 loops Pyrococcus horikoshii