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Literature summary extracted from

  • Zhang, W.; Morris, G.Z.; Beebe, S.J.
    Characterization of the cAMP-dependent protein kinase catalytic subunit Cgamma expressed and purified from sf9 cells (2004), Protein Expr. Purif., 35, 156-169.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.11 cAMP dependent on Mus musculus
2.7.11.11 cAMP dependent on Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.11 expression of His-tagged catalytic subunit Calpha in Escherichia coli strain BL21(DE3), expression of catalytic subunit Calpha in Spodoptera frugiperda Sf9 cells via baculovirus infection system Mus musculus
2.7.11.11 expression of His-tagged catalytic subunit Cgamma in Escherichia coli strain BL21(DE3), stable expression of catalytic subunit Cgamma in cAMP-resistant mouse Y1 adrenal mutant cell line, expression of catalytic subunit Cgamma in Spodoptera frugiperda Sf9 cells via baculovirus infection system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.11 additional information no inhibition of catalytic subunit Cgamma by PKI Homo sapiens
2.7.11.11 PKI catalytic subunit Calpha Mus musculus
2.7.11.11 pseudosubstrate peptides IC50 values for inhibition of the regulatory subunits of PKA by endogenous inhibitors, overview Homo sapiens
2.7.11.11 pseudosubstrate peptides IC50 values for inhibition of he regulatory subunits of PKA by endogenous inhibitors, overview Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.11 additional information
-
 additional information kinetics for catalytic subunit Cgamma Mus musculus
2.7.11.11 additional information
-
 additional information kinetics for catalytic subunit Cgamma Homo sapiens
2.7.11.11 0.0125
-
 Kemptide pH 7.0, 30°C, recombinant catalytic subunit Cgamma Homo sapiens
2.7.11.11 0.0253
-
 RFARKGSLREKNV pH 7.0, 30°C, recombinant catalytic subunit Cgamma Homo sapiens
2.7.11.11 0.0333
-
 RKRSRAE pH 7.0, 30°C, recombinant catalytic subunit Cgamma Homo sapiens
2.7.11.11 0.0333
-
 RKRSRKE pH 7.0, 30°C, recombinant catalytic subunit Cgamma Homo sapiens
2.7.11.11 0.038
-
 Kemptide pH 7.0, 30°C, recombinant catalytic subunit Calpha Mus musculus
2.7.11.11 0.05
-
 RFARKGSLREKNV pH 7.0, 30°C, recombinant catalytic subunit Calpha Mus musculus
2.7.11.11 0.0503
-
 RRLSSLRA pH 7.0, 30°C, recombinant catalytic subunit Cgamma Homo sapiens
2.7.11.11 0.293
-
 RKRSRAE pH 7.0, 30°C, recombinant catalytic subunit Calpha Mus musculus
2.7.11.11 0.338
-
 RRLSSLRA pH 7.0, 30°C, recombinant catalytic subunit Calpha Mus musculus
2.7.11.11 0.5
-
 RKRSRKE pH 7.0, 30°C, recombinant catalytic subunit Calpha Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.11 Mg2+
-
 Mus musculus
2.7.11.11 Mg2+
-
 Homo sapiens
2.7.11.11 Zn2+
-
 Mus musculus
2.7.11.11 Zn2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.11 41000
-
 1 * 41000, isolated recombinant catalytic subunit Cgamma from Sf9 cells, SDS-PAGE Homo sapiens
2.7.11.11 42000
-
 1 * 42000, isolated recombinant catalytic subunit Calpha from Escherichia coli, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Homo sapiens
-
-
-
2.7.11.11 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.11 recombinant catalytic subunit Calpha from Escherichia coli by nickel affinity chromatography, recombinant catalytic subunit Calpha from Sf9 insect cells 62fold by adsorption chromatography and gel filtration Mus musculus
2.7.11.11 recombinant His-tagged catalytic subunit Cgamma from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant catalytic subunit Cgamma 273fold from Sf9 insect cells by adsorption chromatography and gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.11 0.31
-
 purified recombinant catalytic subunit Cgamma, substrate Kemptide Homo sapiens
2.7.11.11 0.81
-
 purified recombinant catalytic subunit Cgamma, substrate histone Homo sapiens
2.7.11.11 5.3
-
 purified recombinant catalytic subunit Calpha Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.7.11.11 -70°C, purified recombinant catalytic subunit Cgamma, 0.5 M NaCl, 10 mM potassium phosphate, pH 6.9, 1 mM EDTA, 30% glycerol, on freeze-thaw cycle, stable up to one year Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 ATP + histone 3s activity of the catalytic subunit Cgamma Homo sapiens ADP + phosphorylated histone 3s
-
 ?
2.7.11.11 ATP + histone IIIS activity of the catalytic subunit Cgamma Mus musculus ADP + phosphorylated histone IIIS
-
 ?
2.7.11.11 ATP + Kemptide activity of the catalytic subunit Calpha Mus musculus ADP + Kemptide phosphate
-
 ?
2.7.11.11 ATP + Kemptide PKA-derived substrate, activity of the catalytic subunit Cgamma Homo sapiens ADP + Kemptide phosphate
-
 ?
2.7.11.11 ATP + RFARKGSLREKNV protein kinase C-derived peptide, activity of the catalytic subunit Calpha Mus musculus ADP + RFARKG-phosphoserine-LREKNV
-
 ?
2.7.11.11 ATP + RFARKGSLREKNV protein kinase C-derived peptide, activity of the catalytic subunit Cgamma Homo sapiens ADP + RFARKG-phosphoserine-LREKNV
-
 ?
2.7.11.11 ATP + RKRSRAE cGPK-1-derived peptide, activity of the catalytic subunit Calpha Mus musculus ADP + RKR-phosphoserine-RAE
-
 ?
2.7.11.11 ATP + RKRSRAE cGPK-1-derived peptide, activity of the catalytic subunit Cgamma Homo sapiens ADP + RKR-phosphoserine-RAE
-
 ?
2.7.11.11 ATP + RKRSRKE cGPK-2-derived peptide, activity of the catalytic subunit Calpha Mus musculus ADP + RKR-phosphoserine-RKE
-
 ?
2.7.11.11 ATP + RKRSRKE cGPK-2-derived peptide, activity of the catalytic subunit Cgamma Homo sapiens ADP + RKR-phosphoserine-RKE
-
 ?
2.7.11.11 ATP + RRLSSLRA S6 kinase-derived peptide, activity of the catalytic subunit Calpha Mus musculus ADP + RRL-phosphoserine-phosphoserine-LRA
-
 ?
2.7.11.11 ATP + RRLSSLRA S6 kinase-derived peptide, activity of the catalytic subunit Cgamma Homo sapiens ADP + RRL-phosphoserine-phosphoserine-LRA
-
 ?
2.7.11.11 additional information substrate specificity, overview Mus musculus ?
-
 ?
2.7.11.11 additional information substrate specificity, overview Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.11 monomer 1 * 41000, isolated recombinant catalytic subunit Cgamma from Sf9 cells, SDS-PAGE Homo sapiens
2.7.11.11 monomer 1 * 42000, isolated recombinant catalytic subunit Calpha from Escherichia coli, SDS-PAGE Mus musculus
2.7.11.11 More determination of Stoke's radius of purified recombinant catalytic subunit Calpha Mus musculus
2.7.11.11 More determination of Stoke's radius of purified recombinant catalytic subunit Cgamma Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
-
 Mus musculus
2.7.11.11 PKA
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.11 30
-
 assay at Mus musculus
2.7.11.11 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.11 7
-
 assay at Mus musculus
2.7.11.11 7
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP
-
 Mus musculus
2.7.11.11 ATP
-
 Homo sapiens