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Literature summary extracted from

  • Wei, W.; Li, H.; Nemeria, N.; Jordan, F.
    Expression and purification of the dihydrolipoamide acetyltransferase and dihydrolipoamide dehydrogenase subunits of the Escherichia coli pyruvate dehydrogenase multienzyme complex: a mass spectrometric assay for reductive acetylation of dihydrolipoamide acetyltransferase (2003), Protein Expr. Purif., 28, 140-150.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.12 functional overexpression of the enzyme E2 with a single hybrid lipoyl domain per subunit, i.e. 1-lip E2, in strain JRG1342 and BL21(DE3), overexpression of the isolated hybrid lipoyl domain in strain JM 101 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.12 Mg2+
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.12 8982
-
 x * 45953, recombinant 1-lip E2, mass spectrometry, x * 8982, recombinant unacetylated hybrid lipoyl domain, mass spectrometry, x * 9019, recombinant fully acetylated hybrid lipoyl domain, mass spectrometry Escherichia coli
2.3.1.12 9019
-
 x * 45953, recombinant 1-lip E2, mass spectrometry, x * 8982, recombinant unacetylated hybrid lipoyl domain, mass spectrometry, x * 9019, recombinant fully acetylated hybrid lipoyl domain, mass spectrometry Escherichia coli
2.3.1.12 45953
-
 x * 45953, recombinant 1-lip E2, mass spectrometry, x * 8982, recombinant unacetylated hybrid lipoyl domain, mass spectrometry, x * 9019, recombinant fully acetylated hybrid lipoyl domain, mass spectrometry Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.12 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.12 recombinant 1-lip E2 and recombinant hybrid lipoyl domain by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.12 reconstitution of the active multienzyme complex with recombinant components Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.12 34.1
-
 purified 1-lip E2 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.12 acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine reductive acetylation of the enzyme Escherichia coli CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.12 ? x * 45953, recombinant 1-lip E2, mass spectrometry, x * 8982, recombinant unacetylated hybrid lipoyl domain, mass spectrometry, x * 9019, recombinant fully acetylated hybrid lipoyl domain, mass spectrometry Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.3.1.12 dihydrolipoamide acetyltransferase
-
 Escherichia coli
2.3.1.12 E2
-
 Escherichia coli
2.3.1.12 More the enzyme is a subunit of the pyruvate dehydrogenase multienzyme complex Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.12 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.12 8
-
 assay at Escherichia coli