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Literature summary extracted from

  • Resnick, A.C.; Snowman, A.M.; Kang, B.N.; Hurt, K.J.; Snyder, S.H.; Saiardi, A.
    Inositol polyphosphate multikinase is a nuclear PI3-kinase with transcriptional regulatory activity (2005), Proc. Natl. Acad. Sci. USA, 102, 12783-12788.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.127 expression of HA-tagged enzyme in human HEK293 and in COS-7 cells Rattus norvegicus
2.7.1.151 expression of HA-tagged enzyme in human HEK293 and in COS-7 cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.151 additional information no inhibition by wortmannin and LY294002 Rattus norvegicus
2.7.1.151 additional information no inhibition by wortmannin and LY294002 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.151 0.006
-
 1D-myo-inositol 4,5-bisphosphate pH 7.4, 37°C Rattus norvegicus
2.7.1.151 0.03
-
 1D-myo-inositol 4,5-bisphosphate pH 7.4, 30°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.127 nucleus
-
 Rattus norvegicus 5634
-
2.7.1.127 nucleus
-
 Saccharomyces cerevisiae 5634
-
2.7.1.151 nucleus
-
 Rattus norvegicus 5634
-
2.7.1.151 nucleus
-
 Saccharomyces cerevisiae 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.127 Mg2+
-
 Rattus norvegicus
2.7.1.127 Mg2+
-
 Saccharomyces cerevisiae
2.7.1.151 Mg2+
-
 Rattus norvegicus
2.7.1.151 Mg2+
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.127 44000
-
 x * 44000, SDS-PAGE Rattus norvegicus
2.7.1.151 44000
-
 x * 44000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.151 ATP + 1D-myo-inositol 4,5-bisphosphate Rattus norvegicus
-
 ADP + 1D-myo-inositol 3,4,5-trisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 4,5-bisphosphate Saccharomyces cerevisiae
-
 ADP + 1D-myo-inositol 3,4,5-trisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 4,5-bisphosphate Saccharomyces cerevisiae BY4741
-
 ADP + 1D-myo-inositol 3,4,5-trisphosphate
-
 ?
2.7.1.151 additional information Saccharomyces cerevisiae the enzyme is involved inregulation of arginine-sensitive ArgR-Mcm1 transcriptional complex, overview ?
-
 ?
2.7.1.151 additional information Saccharomyces cerevisiae BY4741 the enzyme is involved inregulation of arginine-sensitive ArgR-Mcm1 transcriptional complex, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.127 Rattus norvegicus
-
-
-
2.7.1.127 Saccharomyces cerevisiae
-
-
-
2.7.1.127 Saccharomyces cerevisiae BY4741
-
-
-
2.7.1.151 Rattus norvegicus
-
-
-
2.7.1.151 Saccharomyces cerevisiae
-
 wild-type strain BY4741 and several mutant strains
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.127 native enzyme partially by nuclear fraction preparation Rattus norvegicus
2.7.1.127 native enzyme partially by nuclear fraction preparation Saccharomyces cerevisiae
2.7.1.151 native enzyme partially by nuclear fraction preparation Rattus norvegicus
2.7.1.151 native enzyme partially by nuclear fraction preparation Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.127 brain primary hippocampal and corical cultures Rattus norvegicus
-
2.7.1.127 cell culture
-
 Rattus norvegicus
-
2.7.1.127 hepatocyte primary Rattus norvegicus
-
2.7.1.127 liver
-
 Rattus norvegicus
-
2.7.1.151 brain primary hippocampal and corical cultures Rattus norvegicus
-
2.7.1.151 cell culture
-
 Rattus norvegicus
-
2.7.1.151 hepatocyte primary Rattus norvegicus
-
2.7.1.151 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.151 0.5
-
 partially purified enzyme Rattus norvegicus
2.7.1.151 0.7
-
 partially purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol phosphate 3-kinase reaction of the inositol polyphosphate multikinase, EC 2.7.1.151 Rattus norvegicus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol phosphate 3-kinase reaction of the inositol polyphosphate multikinase, EC 2.7.1.151 Saccharomyces cerevisiae ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.127 ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol phosphate 3-kinase reaction of the inositol polyphosphate multikinase, EC 2.7.1.151 Saccharomyces cerevisiae BY4741 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 4,5-bisphosphate
-
 Rattus norvegicus ADP + 1D-myo-inositol 3,4,5-trisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 4,5-bisphosphate
-
 Saccharomyces cerevisiae ADP + 1D-myo-inositol 3,4,5-trisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 4,5-bisphosphate the enzyme is specific for 1D-myo-inositol 4,5-bisphosphate Rattus norvegicus ADP + 1D-myo-inositol 3,4,5-trisphosphate product identification ?
2.7.1.151 ATP + 1D-myo-inositol 4,5-bisphosphate the enzyme is specific for 1D-myo-inositol 4,5-bisphosphate Saccharomyces cerevisiae ADP + 1D-myo-inositol 3,4,5-trisphosphate product identification ?
2.7.1.151 ATP + 1D-myo-inositol 4,5-bisphosphate
-
 Saccharomyces cerevisiae BY4741 ADP + 1D-myo-inositol 3,4,5-trisphosphate
-
 ?
2.7.1.151 ATP + 1D-myo-inositol 4,5-bisphosphate the enzyme is specific for 1D-myo-inositol 4,5-bisphosphate Saccharomyces cerevisiae BY4741 ADP + 1D-myo-inositol 3,4,5-trisphosphate product identification ?
2.7.1.151 additional information the enzyme is involved inregulation of arginine-sensitive ArgR-Mcm1 transcriptional complex, overview Saccharomyces cerevisiae ?
-
 ?
2.7.1.151 additional information the enzyme also performs the inositol phosphate 3-kinase reaction, EC 2.7.1.127 Rattus norvegicus ?
-
 ?
2.7.1.151 additional information the enzyme performs also the inositol phosphate 3-kinase reaction, EC 2.7.1.127 Saccharomyces cerevisiae ?
-
 ?
2.7.1.151 additional information the enzyme is involved inregulation of arginine-sensitive ArgR-Mcm1 transcriptional complex, overview Saccharomyces cerevisiae BY4741 ?
-
 ?
2.7.1.151 additional information the enzyme performs also the inositol phosphate 3-kinase reaction, EC 2.7.1.127 Saccharomyces cerevisiae BY4741 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.127 ? x * 44000, SDS-PAGE Rattus norvegicus
2.7.1.151 ? x * 44000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.127 Arg82
-
 Saccharomyces cerevisiae
2.7.1.127 ArgRIII
-
 Saccharomyces cerevisiae
2.7.1.127 inositol polyphosphate multikinase
-
 Rattus norvegicus
2.7.1.127 inositol polyphosphate multikinase
-
 Saccharomyces cerevisiae
2.7.1.127 Ipk2
-
 Saccharomyces cerevisiae
2.7.1.127 IPMK
-
 Rattus norvegicus
2.7.1.127 IPMK
-
 Saccharomyces cerevisiae
2.7.1.151 Arg82
-
 Saccharomyces cerevisiae
2.7.1.151 ArgRIII
-
 Saccharomyces cerevisiae
2.7.1.151 inositol polyphosphate multikinase
-
 Rattus norvegicus
2.7.1.151 inositol polyphosphate multikinase
-
 Saccharomyces cerevisiae
2.7.1.151 Ipk2
-
 Saccharomyces cerevisiae
2.7.1.151 IPMK
-
 Rattus norvegicus
2.7.1.151 IPMK
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.127 30
-
 assay at Saccharomyces cerevisiae
2.7.1.127 37
-
 assay at Rattus norvegicus
2.7.1.151 30
-
 assay at Saccharomyces cerevisiae
2.7.1.151 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.127 7.4
-
 assay at Rattus norvegicus
2.7.1.127 7.4
-
 assay at Saccharomyces cerevisiae
2.7.1.151 7.4
-
 assay at Rattus norvegicus
2.7.1.151 7.4
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.127 ATP
-
 Rattus norvegicus
2.7.1.127 ATP
-
 Saccharomyces cerevisiae
2.7.1.151 ATP
-
 Rattus norvegicus
2.7.1.151 ATP
-
 Saccharomyces cerevisiae