Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Welin, M.; Kosinska, U.; Mikkelsen, N.E.; Carnrot, C.; Zhu, C.; Wang, L.; Eriksson, S.; Munch-Petersen, B.; Eklund, H.
    Structures of thymidine kinase 1 of human and mycoplasmic origin (2004), Proc. Natl. Acad. Sci. USA, 101, 17970-17975.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.1.21 medicine enzyme can be useful in gene therapy Ureaplasma urealyticum
2.7.1.21 medicine enzyme can be useful in gene therapy Homo sapiens
2.7.1.21 synthesis enzyme can be useful in drug design Ureaplasma urealyticum
2.7.1.21 synthesis enzyme can be useful in drug design Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 expression of C-terminally truncated enzyme lacking 41 amino acid residues Homo sapiens
2.7.1.21 gene tdk, expression in Escherichia coli as wild-type and selenomethionine-labeled enzymes Ureaplasma urealyticum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.21 purified recombinant C-terminally truncated enzyme in complex with inhibitor dTTP, hanging drop vapour diffusion method, 7-13 mg/ml protein in solution with 5 mM dTTP mixed with an equal volume of crystallization solution containing 0.1 M Na-citrate, pH 5.6, 20% 2-propanol, and 14-16% PEG 4000, 15°C, X-ray diffraction structure determination and analysis at 2.4-3.5 A resolution Homo sapiens
2.7.1.21 purified recombinant wild-type and selenomethionine-labeled enzyme in complex with inhibitor dTTP, hanging drop vapour diffusion method, 8 mg/ml protein in solution with 5 mM dTTp mixed with an equal volume of reservoir solution containing 19% PEG 2000, 0.2 M LiCl, 5 mM DTT, and 0.1 M MES, pH 6.0, 15°C, X-ray diffraction structure determination and analysis at 2.5 or 3.0 A resolution, respectively Ureaplasma urealyticum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 dTTP competitive feedback inhibition, binding structure analysis Homo sapiens
2.7.1.21 dTTP competitive feedback inhibition, binding structure analysis Ureaplasma urealyticum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.21 cytosol
-
 Ureaplasma urealyticum 5829
-
2.7.1.21 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.21 Mg2+
-
 Ureaplasma urealyticum
2.7.1.21 Mg2+
-
 Homo sapiens
2.7.1.21 Zn2+ at a molar ratio of 0.51, connects beta-structures at the root of a ribbon that forms a stem which widens to a lasso-loop Ureaplasma urealyticum
2.7.1.21 Zn2+ at a molar ratio of 0.56, connects beta-structures at the root of a ribbon that forms a stem which widens to a lasso-loop Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.21 ATP + 2'-deoxythymidine Ureaplasma urealyticum
-
 ADP + 2'-deoxythymidine 5'-phosphate
-
 ir
2.7.1.21 ATP + 2'-deoxythymidine Homo sapiens
-
 ADP + 2'-deoxythymidine 5'-phosphate
-
 ir
2.7.1.21 ATP + 2'-deoxyuridine Ureaplasma urealyticum
-
 ADP + 2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 2'-deoxyuridine Homo sapiens
-
 ADP + 2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 additional information Homo sapiens the cell-cycle-regulated enzyme is important in nucleotide metabolism and an activator of antiviral and anticancer drugs ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Homo sapiens P04183
-
-
2.7.1.21 Ureaplasma urealyticum
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.21 ATP + thymidine = ADP + dTMP substrate binding and active site structure Ureaplasma urealyticum
a
2.7.1.21 ATP + thymidine = ADP + dTMP substrate binding and active site structure Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + 2'-deoxythymidine
-
 Ureaplasma urealyticum ADP + 2'-deoxythymidine 5'-phosphate
-
 ir
2.7.1.21 ATP + 2'-deoxythymidine
-
 Homo sapiens ADP + 2'-deoxythymidine 5'-phosphate
-
 ir
2.7.1.21 ATP + 2'-deoxyuridine
-
 Ureaplasma urealyticum ADP + 2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 ATP + 2'-deoxyuridine
-
 Homo sapiens ADP + 2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.21 additional information the cell-cycle-regulated enzyme is important in nucleotide metabolism and an activator of antiviral and anticancer drugs Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 tetramer each subunit contains an alpha/beta-domain, and a domain with a structural zinc ion Ureaplasma urealyticum
2.7.1.21 tetramer each subunit contains an alpha/beta-domain, and a domain with a structural zinc ion Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.21 More the enzyme belongs to the thymidine kinase 1, i.e. TK1, family of enzymes Ureaplasma urealyticum
2.7.1.21 More the enzyme belongs to the thymidine kinase 1, i.e. TK1, family of enzymes Homo sapiens
2.7.1.21 thymidine kinase 1
-
 Ureaplasma urealyticum
2.7.1.21 thymidine kinase 1
-
 Homo sapiens
2.7.1.21 TK1
-
 Ureaplasma urealyticum
2.7.1.21 TK1
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.21 ATP
-
 Ureaplasma urealyticum
2.7.1.21 ATP
-
 Homo sapiens