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Literature summary extracted from

  • Tsuji, N.; Nishikori, S.; Iwabe, O.; Matsumoto, S.; Shiraki, K.; Miyasaka, H.; Takagi, M.; Miyamoto, K.; Hirata, K.
    Comparative analysis of the two-step reaction catalyzed by prokaryotic and eukaryotic phytochelatin synthase by an ion-pair liquid chromatography assay (2005), Planta, 222, 181-191.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.15
-
 Nostoc sp.
2.3.2.15
-
 Arabidopsis thaliana
2.3.2.15 expressed in Escherichia coli BL21(DE3) cells Nostoc sp.
2.3.2.15 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.15 DELTA222-485 truncation mutant is fulla sufficient for phytochelatin synthesis. The fragment may be insufficient to maintain the active form of the enzyme stably in vitro Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.15 Cd2+ required, 0.5 mM Nostoc sp.
2.3.2.15 Cd2+ required, 0.5 mM Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.15 Arabidopsis thaliana Q9S7Z3
-
-
2.3.2.15 Nostoc sp.
-
-
-
2.3.2.15 Nostoc sp.
-
 PCC7120
-
2.3.2.15 Nostoc sp. PCC 7120
-
 PCC7120
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.15 DEAE-Toyopearl column chromatography and HiTrap SP column chromatography Nostoc sp.
2.3.2.15 DEAE-Toyopearl column chromatography, HiTrap SP column chromatography, and Hitrap Q sepharose column chromatography Arabidopsis thaliana
2.3.2.15 recombinant enzyme Nostoc sp.
2.3.2.15 recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
 Nostoc sp. Gly + [Glu(-Cys)]n+1-Gly
-
 ?
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
 Arabidopsis thaliana Gly + [Glu(-Cys)]n+1-Gly
-
 ?
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
 Nostoc sp. PCC 7120 Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.15 AtPCS
-
 Arabidopsis thaliana
2.3.2.15 NsPCS
-
 Nostoc sp.
2.3.2.15 PC synthase
-
 Nostoc sp.
2.3.2.15 PC synthase
-
 Arabidopsis thaliana
2.3.2.15 PCS
-
 Nostoc sp.
2.3.2.15 PCS1
-
 Arabidopsis thaliana
2.3.2.15 phytochelatin synthase
-
 Nostoc sp.
2.3.2.15 phytochelatin synthase
-
 Arabidopsis thaliana