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Literature summary extracted from
- Functional characterization of nine Norway spruce TPS genes and evolution of gymnosperm terpene synthases of the TPS-d subfamily (2004), Plant Physiol., 135, 1908-1927.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.3.16 | expression in Escherichia coli | Picea abies |
| 4.2.3.38 | gene PaTPS-Bis, DNA and amino acid sequence determination and analysis, expression and phylogenetic analysis, sequence comparison with other enzymes of the terpene synthase family, functional expression in Escherichia coli | Picea abies |
| 4.2.3.44 | expression in Escherichia coli | Picea abies |
| 4.2.3.80 | - |
Picea abies |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.38 | (2E,6E)-farnesyl diphosphate | Picea abies | a step in the terpene synthesis pathway, overview | (E)-alpha-bisabolene + diphosphate | - |
? |  |
| 4.2.3.58 | (2E,6E)-farnesyl diphosphate | Picea abies | - |
longifolene + diphosphate | main product | ? | |
| 4.2.3.58 | (2E,6E)-farnesyl diphosphate | Picea abies Karst | - |
longifolene + diphosphate | main product | ? | |
| 4.2.3.58 | additional information | Picea abies | longifolene synthase gives besides the main product longifolene (60.6% yield) alpha-longipinene (14.6%), longicyclene (5.9%), (E)-beta-farnesene (3.4%), longiborneol (2.1%), cyclosativene (1.3%), beta-longipinene (1.4%) and other sesquiterpenes (less than 1.4% each) | ? | - |
? | |
| 4.2.3.58 | additional information | Picea abies Karst | longifolene synthase gives besides the main product longifolene (60.6% yield) alpha-longipinene (14.6%), longicyclene (5.9%), (E)-beta-farnesene (3.4%), longiborneol (2.1%), cyclosativene (1.3%), beta-longipinene (1.4%) and other sesquiterpenes (less than 1.4% each) | ? | - |
? | |
| 4.2.3.80 | (2E,6E)-farnesyl diphosphate | Picea abies | - |
alpha-longipinene + diphosphate | - |
? | |
| 4.2.3.80 | (2E,6E)-farnesyl diphosphate | Picea abies Karst | - |
alpha-longipinene + diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.16 | Picea abies | Q675L1 | - |
- |
| 4.2.3.38 | Picea abies | Q675L6 | Norway spruce, gene PaTPS-Bis | - |
| 4.2.3.44 | Picea abies | Q675L5 | L. Karst | - |
| 4.2.3.58 | Picea abies | Q675L0 | Norway spruce | - |
| 4.2.3.80 | Picea abies | Q675L0 | - |
- |
| 4.2.3.80 | Picea abies Karst | Q675L0 | - |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.2.3.44 | (+)-copalyl diphosphate = isopimara-7,15-diene + diphosphate | proposed reaction scheme | Picea abies |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.38 | (2E,6E)-farnesyl diphosphate | - |
Picea abies | (E)-alpha-bisabolene + diphosphate | product identification by GC-MS | ? | |
| 4.2.3.38 | (2E,6E)-farnesyl diphosphate | a step in the terpene synthesis pathway, overview | Picea abies | (E)-alpha-bisabolene + diphosphate | - |
? | |
| 4.2.3.44 | copalyl diphosphate | proposed reaction scheme | Picea abies | isopimara-7,15-diene + diphosphate | - |
? | |
| 4.2.3.58 | (2E,6E)-farnesyl diphosphate | - |
Picea abies | longifolene + diphosphate | main product | ? | |
| 4.2.3.58 | (2E,6E)-farnesyl diphosphate | - |
Picea abies Karst | longifolene + diphosphate | main product | ? | |
| 4.2.3.58 | additional information | longifolene synthase gives besides the main product longifolene (60.6% yield) alpha-longipinene (14.6%), longicyclene (5.9%), (E)-beta-farnesene (3.4%), longiborneol (2.1%), cyclosativene (1.3%), beta-longipinene (1.4%) and other sesquiterpenes (less than 1.4% each) | Picea abies | ? | - |
? | |
| 4.2.3.58 | additional information | longifolene synthase gives besides the main product longifolene (60.6% yield) alpha-longipinene (14.6%), longicyclene (5.9%), (E)-beta-farnesene (3.4%), longiborneol (2.1%), cyclosativene (1.3%), beta-longipinene (1.4%) and other sesquiterpenes (less than 1.4% each) | Picea abies Karst | ? | - |
? | |
| 4.2.3.80 | (2E,6E)-farnesyl diphosphate | - |
Picea abies | alpha-longipinene + diphosphate | - |
? | |
| 4.2.3.80 | (2E,6E)-farnesyl diphosphate | the enzyme from Norway spruce produces longifolene (61%) as the main product (c.f. EC 4.2.3.58, longifolene synthase). alpha-Longipinene constitutes about 15% of the total products | Picea abies | alpha-longipinene + diphosphate | - |
? | |
| 4.2.3.80 | (2E,6E)-farnesyl diphosphate | - |
Picea abies Karst | alpha-longipinene + diphosphate | - |
? | |
| 4.2.3.80 | (2E,6E)-farnesyl diphosphate | the enzyme from Norway spruce produces longifolene (61%) as the main product (c.f. EC 4.2.3.58, longifolene synthase). alpha-Longipinene constitutes about 15% of the total products | Picea abies Karst | alpha-longipinene + diphosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.3.16 | (-)-limonene synthase | - |
Picea abies |
| 4.2.3.38 | E-alpha-bisabolene synthase | - |
Picea abies |
| 4.2.3.38 | More | the enzyme belongs to the sesquiterpene synthase family of enzymes, TPS-d subfamily | Picea abies |
| 4.2.3.44 | PaTPS-Iso | - |
Picea abies |
| 4.2.3.80 | PaTPS-Lon | - |
Picea abies |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.2.3.44 | Picea abies | calculated from sequence | - |
5.8 |
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