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Literature summary extracted from

  • Sauter, M.; Lorbiecke, R.; Ouyang, B.; Pochapsky, T.C.; Rzewuski, G.
    The immediate-early ethylene response gene OsARD1 encodes an acireductone dioxygenase involved in recycling of the ethylene precursor S-adenosylmethionine (2005), Plant J., 44, 718-729.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.53 ethylene
-
 Oryza sativa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.53 expressed in Escherichia coli BL21 cells Oryza sativa
1.13.11.54 expression in Escherichia coli Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.53 cycloheximide 0.0711 mM or higher Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.53 0.044
-
 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one
-
 Oryza sativa
1.13.11.54 1.1
-
 O2
-
 Oryza sativa
1.13.11.54 25.9
-
 1,2-dihydroxy-3-oxopent-1-ene
-
 Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.53 Ni2+
-
 Oryza sativa
1.13.11.54 Fe2+ bacterially expressed AsARD1 preferentially binds Fe2+ rather than Ni2+ Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.54 additional information Oryza sativa OsARD1 is a primary ethylene response gene. Enzyme catalyzes the penultimate step in the methionine cycle. ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.53 Oryza sativa A2Z7C4 subspecies Oryza sativa indica
-
1.13.11.54 Oryza sativa
-
 indica cultivar Pin Gaew 56
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.53 DEAE cellulose column chromatography and Phenyl-Sepharose column chromatography Oryza sativa
1.13.11.54 recombinant Oryza sativa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.13.11.53 unfolded using 8 mM urea and refolded in the presence of 20 mM Ni2+ Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.53 internode
-
 Oryza sativa
-
1.13.11.53 root
-
 Oryza sativa
-
1.13.11.54 internode strongly induced in adventitious roots and in the youngest internode of partially submerged plants Oryza sativa
-
1.13.11.54 root strongly induced in adventitious roots and in the youngest internode of partially submerged plants Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.53 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
-
 Oryza sativa 3-(methylthio)propanoate + formate + CO
-
 ?
1.13.11.54 1,2-dihydroxy-3-oxopent-1-ene + O2
-
 Oryza sativa 2-oxo-butanoate + formate
-
 ?
1.13.11.54 additional information OsARD1 is a primary ethylene response gene. Enzyme catalyzes the penultimate step in the methionine cycle. Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.53 oligomer
-
 Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
1.13.11.53 ARD1
-
 Oryza sativa
1.13.11.54 OsARD1
-
 Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.54 11.7
-
 1,2-dihydroxy-3-oxopent-1-ene
-
 Oryza sativa
1.13.11.54 11.8
-
 O2
-
 Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.53 7.4
-
-
 Oryza sativa