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Literature summary extracted from

  • Luecker, J.; Bowen, P.; Bohlmann, J.
    Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries (2004), Phytochemistry, 65, 2649-2659.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.73 expression in Escherichia coli Vitis vinifera
4.2.3.75 DNA and amino acid sequence determination and analysis of Vitis vinifera cv. Gewürztraminer, phylogenetic tree, expression in Escherichia coli strain BL21(DE3)-RIL Vitis vinifera
4.2.3.75 expression in Escherichia coli Vitis vinifera
4.2.3.86 expression in Escherichia coli Vitis vinifera

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.75 Mg2+
-
 Vitis vinifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.73 64400
-
 x * 64400, calculated Vitis vinifera
4.2.3.75 64300
-
 x * 64300, calculated Vitis vinifera
4.2.3.86 64400
-
 x * 64400, calculated from sequence Vitis vinifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.73 farnesyl diphosphate Vitis vinifera
-
 (+)-valencene + diphosphate 49.5% (+)-valencene and 35.5% (-)-7-epi-alpha-selinene, plus minor amounts of alpha-selinene, epi-aristolochene, E-beta-caryophyllene, alpha-humulene ?
4.2.3.73 farnesyl diphosphate Vitis vinifera cv. Gewuerztraminer
-
 (+)-valencene + diphosphate 49.5% (+)-valencene and 35.5% (-)-7-epi-alpha-selinene, plus minor amounts of alpha-selinene, epi-aristolochene, E-beta-caryophyllene, alpha-humulene ?
4.2.3.75 2-trans,6-trans-farnesyl diphosphate Vitis vinifera
-
 (-)-(7S)-germacrene D + diphosphate
-
 ?
4.2.3.75 additional information Vitis vinifera cell terpenoid profile determination ?
-
 ?
4.2.3.86 (2E,6E)-farnesyl diphosphate Vitis vinifera the recombinant enzyme from Vitis vinifera forms 49.5% (+)-valencene (cf EC 4.2.3.73, valencene synthase) and 35.5% (-)-7-epi-alpha-selinene 7-epi-alpha-selinene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.73 Vitis vinifera
-
-
-
4.2.3.73 Vitis vinifera Q6Q3H2
-
-
4.2.3.73 Vitis vinifera cv. Gewuerztraminer
-
-
-
4.2.3.75 Vitis vinifera Q6Q3H3
-
-
4.2.3.75 Vitis vinifera Q6Q3H3 cv. Chardonnay and cv. Gewürztraminer
-
4.2.3.86 Vitis vinifera Q6Q3H2 cv. Chardonnay and cv. Gewürztraminer
-
4.2.3.111 Vitis vinifera
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.73 flower strongest hybridization signals with RNA from flower budsand strongly reduced transcript levels in pre- and postanthesis open flowers and in set flowers at early fruit onset Vitis vinifera
-
4.2.3.73 fruit transcripts become detectable in September, almost two months after flowering, then increase and reach a maximum at the final sample date in early October when the fruit is at peak maturity Vitis vinifera
-
4.2.3.73 fruit detection of VvVal transcripts coincides with the stabilization of acid levels in the fruits following a four-week period of rapid depletion Vitis vinifera
-
4.2.3.73 leaf vegetative tissues of young leaves Vitis vinifera
-
4.2.3.73 additional information transcript levels are low in open pre-anthesis flowers, flowers after anthesis, or at early onset of fruit development Vitis vinifera
-
4.2.3.73 stem green stem Vitis vinifera
-
4.2.3.73 tendril
-
 Vitis vinifera
-
4.2.3.75 flower
-
 Vitis vinifera
-
4.2.3.75 flower strongest hybridization signals with RNA from flower budsand strongly reduced transcript levels in pre- and postanthesis open flowers and in set flowers at early fruit onset Vitis vinifera
-
4.2.3.75 flower bud
-
 Vitis vinifera
-
4.2.3.75 fruit young Vitis vinifera
-
4.2.3.75 leaf vegetative tissues of young leaves Vitis vinifera
-
4.2.3.75 additional information no transcripts in fruit Vitis vinifera
-
4.2.3.75 stem green stem Vitis vinifera
-
4.2.3.75 tendril
-
 Vitis vinifera
-
4.2.3.86 flower transcript levels are low in open pre-anthesis flowers, flowers after anthesis, or at early onset of fruit development Vitis vinifera
-
4.2.3.86 fruit transcripts become detectable in September, almost two months after flowering, then increase and reach a maximum at the final sample date in early October when the fruit is at peak maturity Vitis vinifera
-
4.2.3.111 flower strongest signals with RNA from flower buds and strongly reduced transcript levels in pre- and postanthesis open flowers and in set flowers at early fruit onset Vitis vinifera
-
4.2.3.111 additional information expression appears restricted to flowers Vitis vinifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.73 (2E,6E)-farnesyl diphosphate
-
 Vitis vinifera (+)-valencene + diphosphate 49.5% (+)-valencene and 35.5% (-)-7-epi-alpha-selinene, plus minor amounts of alpha-selinene, epi-aristolochene, E-beta-caryophyllene, alpha-humulene ?
4.2.3.73 (2E,6E)-farnesyl diphosphate
-
 Vitis vinifera (+)-valencene + diphosphate about 49%, plus 35.5% (-)-7-epi-alpha-selinene ?
4.2.3.73 (2E,6E)-farnesyl diphosphate
-
 Vitis vinifera cv. Gewuerztraminer (+)-valencene + diphosphate 49.5% (+)-valencene and 35.5% (-)-7-epi-alpha-selinene, plus minor amounts of alpha-selinene, epi-aristolochene, E-beta-caryophyllene, alpha-humulene ?
4.2.3.73 farnesyl diphosphate
-
 Vitis vinifera (+)-valencene + diphosphate 49.5% (+)-valencene and 35.5% (-)-7-epi-alpha-selinene, plus minor amounts of alpha-selinene, epi-aristolochene, E-beta-caryophyllene, alpha-humulene ?
4.2.3.73 farnesyl diphosphate
-
 Vitis vinifera cv. Gewuerztraminer (+)-valencene + diphosphate 49.5% (+)-valencene and 35.5% (-)-7-epi-alpha-selinene, plus minor amounts of alpha-selinene, epi-aristolochene, E-beta-caryophyllene, alpha-humulene ?
4.2.3.73 additional information no substrate: geranyl diphosphate or geranylgeranyl diphosphate Vitis vinifera ?
-
 ?
4.2.3.73 additional information no substrate: geranyl diphosphate or geranylgeranyl diphosphate Vitis vinifera cv. Gewuerztraminer ?
-
 ?
4.2.3.75 (2E,6E)-farnesyl diphosphate
-
 Vitis vinifera (-)-germacrene D + diphosphate plus about 8% dleta-cadinene ?
4.2.3.75 2-trans,6-trans-farnesyl diphosphate
-
 Vitis vinifera (-)-(7S)-germacrene D + diphosphate
-
 ?
4.2.3.75 2-trans,6-trans-farnesyl diphosphate strictly enantio-specific reaction of (-)-germacrene D synthase Vitis vinifera (-)-(7S)-germacrene D + diphosphate GC-MS product analysis ?
4.2.3.75 additional information cell terpenoid profile determination Vitis vinifera ?
-
 ?
4.2.3.75 additional information no substrate: geranyl diphosphate or geranylgeranyl diphosphate Vitis vinifera ?
-
 ?
4.2.3.86 (2E,6E)-farnesyl diphosphate the recombinant enzyme from Vitis vinifera forms 49.5% (+)-valencene (cf EC 4.2.3.73, valencene synthase) and 35.5% (-)-7-epi-alpha-selinene Vitis vinifera 7-epi-alpha-selinene + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.73 ? x * 64400, calculated Vitis vinifera
4.2.3.75 ? x * 64300, calculated Vitis vinifera
4.2.3.86 ? x * 64400, calculated from sequence Vitis vinifera

Synonyms

EC Number Synonyms Comment Organism
4.2.3.73 Val
-
 Vitis vinifera
4.2.3.73 VitiM4670
-
 Vitis vinifera
4.2.3.73 VvVal
-
 Vitis vinifera
4.2.3.75 (-)-germacrene D synthase
-
 Vitis vinifera
4.2.3.75 GerD
-
 Vitis vinifera
4.2.3.75 terpenoid cyclase
-
 Vitis vinifera
4.2.3.75 TPS
-
 Vitis vinifera
4.2.3.75 VitiM1610
-
 Vitis vinifera
4.2.3.86 VvVal
-
 Vitis vinifera

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.75 30
-
 assay at Vitis vinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.75 7
-
 assay at Vitis vinifera

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.3.73 Vitis vinifera calculated
-
 5.8
4.2.3.75 Vitis vinifera calculated
-
 4.5
4.2.3.86 Vitis vinifera calculated
-
 5.8

Expression

EC Number Organism Comment Expression
4.2.3.73 Vitis vinifera transcript levels are low in open pre-anthesis flowers, flowers after anthesis, or at early onset of fruit development down
4.2.3.86 Vitis vinifera transcript levels are low in open pre-anthesis flowers, flowers after anthesis, or at early onset of fruit development down