EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.1.145 | medicine | the enzyme can be used as a suicide gene in combined gene/chemotherapy treatment of cancer | Drosophila melanogaster |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.145 | expression of wild-type and mutant enzymes as glutathione-S-transferase fusion proteins in Escherichia coli | Drosophila melanogaster |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.145 | M88R | site-directed mutagenesis of the full length enzyme and of the DELTA20 N-terminally truncated enzyme, highly increased Km for acceptor substrates, altered substrate specificity compared to the wild-type enzyme | Drosophila melanogaster |
2.7.1.145 | V84A/M88R | site-directed mutagenesis of the full length enzyme and of the DELTA20 N-terminally truncated enzyme, highly increased Km for acceptor substrates, altered substrate specificity compared to the wild-type enzyme | Drosophila melanogaster |
2.7.1.145 | V84A/M88R/A110D | site-directed mutagenesis of the full length enzyme and of the DELTA20 N-terminally truncated enzyme, highly increased Km for acceptor substrates, altered substrate specificity compared to the wild-type enzyme | Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.145 | 0.0025 | - |
2'-deoxythymidine | recombinant wild-type enzyme, pH 7.6, 37°C | Drosophila melanogaster | |
2.7.1.145 | 0.79 | - |
2'-deoxythymidine | recombinant N-terminally truncated mutant M88R, pH 7.6, 37°C | Drosophila melanogaster | |
2.7.1.145 | 0.92 | - |
ganciclovir | recombinant mutant M88R, pH 7.6, 37°C | Drosophila melanogaster | |
2.7.1.145 | 0.93 | - |
2'-deoxythymidine | recombinant mutant M88R, pH 7.6, 37°C | Drosophila melanogaster | |
2.7.1.145 | 1.2 | - |
ganciclovir | recombinant N-terminally truncated mutant M88R, pH 7.6, 37°C | Drosophila melanogaster | |
2.7.1.145 | 1.75 | - |
ganciclovir | recombinant N-terminally truncated mutant V84A/M88R, pH 7.6, 37°C | Drosophila melanogaster | |
2.7.1.145 | 2.68 | - |
ganciclovir | recombinant mutant V84A/M88R, pH 7.6, 37°C | Drosophila melanogaster | |
2.7.1.145 | 3.3 | - |
2'-deoxythymidine | recombinant N-terminally truncated mutant V84A/M88R, pH 7.6, 37°C | Drosophila melanogaster | |
2.7.1.145 | 5.02 | - |
2'-deoxythymidine | recombinant mutant V84A/M88R, pH 7.6, 37°C | Drosophila melanogaster | |
2.7.1.145 | 5.06 | - |
ganciclovir | recombinant mutant V84A/M88R/A110D, pH 7.6, 37°C | Drosophila melanogaster |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.145 | Mg2+ | - |
Drosophila melanogaster |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.145 | Drosophila melanogaster | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.145 | recombinant wild-type and mutant enzymes as glutathione-S-transferase fusion proteins from Escherichia coli by glutathione affinity chromatography | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.145 | ATP + 1-beta-D-arabinofuranosylguanine | poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R | Drosophila melanogaster | ADP + 1-beta-D-arabinofuranosylguanine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2',2'-difluorodeoxycytidine | best substrate for the N-terminally truncated mutant M88R | Drosophila melanogaster | ADP + 2',2'-difluorodeoxycytidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2',2'-difluorodeoxyguanosine | poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R | Drosophila melanogaster | ADP + 2',2'-difluorodeoxyguanosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyadenosine | best substrate for the N-terminally truncated mutant V84A/M88R | Drosophila melanogaster | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyguanosine | poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R | Drosophila melanogaster | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxythymidine | best substrate for the wild-type enzyme | Drosophila melanogaster | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 9-beta-D-arabinofuranosyladenine | poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R | Drosophila melanogaster | ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + E-5-(2-bromovinyl)-2'-deoxyuridine | - |
Drosophila melanogaster | ADP + E-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + ganciclovir | poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R | Drosophila melanogaster | ADP + ganciclovir phosphate | - |
? | |
2.7.1.145 | additional information | broad substrate specificity | Drosophila melanogaster | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.145 | deoxyribonucleoside kinase | - |
Drosophila melanogaster |
2.7.1.145 | dNK | - |
Drosophila melanogaster |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.145 | 37 | - |
assay at | Drosophila melanogaster |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.145 | 7.6 | - |
assay at | Drosophila melanogaster |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.145 | ATP | - |
Drosophila melanogaster |