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Literature summary extracted from

  • Jain, P.; Nagaraja, V.
    An atypical type II topoisomerase from Mycobacterium smegmatis with positive supercoiling activity (2005), Mol. Microbiol., 58, 1392-1405.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.2 enzyme is encoded by 2 orfs TopoM and TopoN, DNA sequence determination and analysis, phylogenetic analysis Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.2 Ciprofloxacin weak inhibition of TopoNM with IC50 of 0.125 mg/ml Mycolicibacterium smegmatis
5.6.2.2 novobiocin inhibition of TopoNM with IC50 of 0.075 mg/ml Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Mycolicibacterium smegmatis
-
 encoded by 2 orfs TopoM and TopoN
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.2 by ion exchange chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA Mycolicibacterium smegmatis ?
-
 ?
5.6.2.2 additional information the enzyme introduces positively supercoils in both relaxed and negatively supercoiled DNA in an ATP-dependent manner Mycolicibacterium smegmatis ?
-
 ?
5.6.2.2 negatively supercoiled DNA + ATP + H2O ATP-dependent relaxation of negatively supercoiled DNA Mycolicibacterium smegmatis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.2.2 More domain organization Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
5.6.2.2 TopoNM
-
 Mycolicibacterium smegmatis
5.6.2.2 type II topoisomerase
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
5.6.2.2 ATP
-
 Mycolicibacterium smegmatis