EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.10.1 | expression of wild-type FLT-3 and mutant FLT-3 ITD in HEK293 cells, expression of hemagglutinin-tagged wild-type FLT-3, containing an ER-targeting sequence, in THP-1 cells, expression of hemagglutinin-tagged or EGFP-tagged, connected via a six-glycine-linker, FLT-3 in COS-7 cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.10.1 | K634A | site-directed mutagenesis PDGFbeta receptor tyrosine kinase, results in impaired tyrosine autophosphorylation and maturation, as well as loss of kinase activity | Homo sapiens |
2.7.10.1 | K644A | site-directed mutagenesis of juxtamembrane tandem mutagenic construct of FLT-3 RTK and of wild-type FLT-3 RTK, maturation of the mutants by glycosylation and phosphorylation is impaired, altered localization compaired to the wild-type enzyme, overview | Homo sapiens |
2.7.10.1 | additional information | construction of FLT-3 with a tandem repeat of the juxtamembrane, termed FLT-3 ITD RTK, showing impaired maturation during recombinant expression in MV4-11 cells, the mutation alters enzyme interactions with other proteins, e.g. heat shock proteins, overview | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.10.1 | AG1296 | FLT-3 inhibitor treatment results in formation of larger amounts of the mature 150 kDa form of mutant FLT-3 ITD | Homo sapiens | |
2.7.10.1 | SU11248 | FLT-3 inhibitor treatment results in formation of larger amounts of the mature 150 kDa form of mutant FLT-3 ITD | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.10.1 | membrane | - |
Homo sapiens | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.10.1 | 130000 | - |
x * 130000, recombinant FLT-3 ITD mutant, SDS-PAGE, x * 150000, recombinant wild-type FLT-3, SDS-PAGE | Homo sapiens |
2.7.10.1 | 150000 | - |
x * 130000, recombinant FLT-3 ITD mutant, SDS-PAGE, x * 150000, recombinant wild-type FLT-3, SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.10.1 | ATP + a [protein]-L-tyrosine | Homo sapiens | FLT-3 is involved in signal transduction, mechanisms | ADP + a [protein]-L-tyrosine phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.10.1 | Homo sapiens | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.10.1 | glycoprotein | FLT-3 and FLT-3 ITD are high-mannose-type glycoproteins | Homo sapiens |
2.7.10.1 | phosphoprotein | tyrosine autophosphorylation regulates maturation of the FLT-3 RTK and of PDGFbeta receptor | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.10.1 | ATP + a [protein]-L-tyrosine | - |
Homo sapiens | ADP + a [protein]-L-tyrosine phosphate | - |
? | |
2.7.10.1 | ATP + a [protein]-L-tyrosine | FLT-3 is involved in signal transduction, mechanisms | Homo sapiens | ADP + a [protein]-L-tyrosine phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.10.1 | ? | x * 130000, recombinant FLT-3 ITD mutant, SDS-PAGE, x * 150000, recombinant wild-type FLT-3, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.10.1 | FLT-3 | - |
Homo sapiens |
2.7.10.1 | fms-like tyrosine kinase 3 | - |
Homo sapiens |
2.7.10.1 | receptor tyrosine kinase | - |
Homo sapiens |
2.7.10.1 | RTK | - |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.10.1 | ATP | - |
Homo sapiens |