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Literature summary extracted from

  • Andreoni, F.; Serafini, G.; Laguardia, M.E.; Magnani, M.
    Bovine hexokinase type I: full-length cDNA sequence and characterisation of the recombinant enzyme (2005), Mol. Cell. Biochem., 268, 9-18.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 type I enzyme, DNA and amino acid sequence determination and analysis, functional expression of soluble full length enzyme and of the soluble C-terminal domain in Escherichia coli strain BL21(DE3) Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 D-Glucose 1,6-bisphosphate competitive versus MgATP2-, low concentration of phosphate counteract Bos taurus
2.7.1.1 D-glucose 6-phosphate competitive versus MgATP2- Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.047
-
 D-glucose pH 7.2, 37°C, recombinant catalytic C-terminal domain Bos taurus
2.7.1.1 0.072
-
 D-glucose pH 7.2, 37°C, recombinant full length enzyme Bos taurus
2.7.1.1 0.67
-
 ATP pH 7.2, 37°C, recombinant full length enzyme Bos taurus
2.7.1.1 0.67
-
 ATP ATP in form of MgATP2- Bos taurus
2.7.1.1 0.98
-
 ATP pH 7.2, 37°C, recombinant catalytic C-terminal domain Bos taurus
2.7.1.1 0.98
-
 ATP ATP in form of MgATP2- Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.1 Mg2+ MgATP2- Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 56000
-
 x * 111000, recombinant full length enzyme, SDS-PAGE, x * 56000, recombinant catalytic C-terminal domain, SDS-PAGE Bos taurus
2.7.1.1 111000
-
 x * 111000, recombinant full length enzyme, SDS-PAGE, x * 56000, recombinant catalytic C-terminal domain, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.1 ATP + D-glucose Bos taurus involved in glucose catabolism ADP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Bos taurus Q5W5U3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 recombinant full length enzyme and C-terminal domain from Escherichia coli strain BL21(DE3) by ion exchange, hydrophobic interaction, anddye ligand affinity chromatography, and gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 1.44
-
 cell extract of recombinant Escherichia coli expressing the full length enzyme Bos taurus
2.7.1.1 3.5
-
 cell extract of recombinant Escherichia coli expressing the catalytic C-terminal domain of the enzyme Bos taurus
2.7.1.1 94
-
 purified recombinant full length enzyme Bos taurus
2.7.1.1 141
-
 purified recombinant catalytic C-terminal domain of the enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + 2-deoxy-2-fluoro-D-glucose
-
 Bos taurus ADP + 2-deoxy-2-fluoro-D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucose
-
 Bos taurus ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucose involved in glucose catabolism Bos taurus ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 additional information the active site is located in the C-terminal domain Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.1 ? x * 111000, recombinant full length enzyme, SDS-PAGE, x * 56000, recombinant catalytic C-terminal domain, SDS-PAGE Bos taurus
2.7.1.1 More the active site is located in the C-terminal domain Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.1 hexokinase type I
-
 Bos taurus
2.7.1.1 HK1
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 8
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.1 6 9
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.1 ATP MgATP2- Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.1 0.033
-
 D-Glucose 1,6-bisphosphate pH 7.2, 37°C, recombinant catalytic C-terminal domain, versus MgATP2- Bos taurus
2.7.1.1 0.036
-
 D-Glucose 1,6-bisphosphate pH 7.2, 37°C, recombinant full length enzyme, versus MgATP2- Bos taurus
2.7.1.1 0.045
-
 D-glucose 6-phosphate pH 7.2, 37°C, recombinant full length enzyme Bos taurus
2.7.1.1 0.066
-
 D-glucose 6-phosphate pH 7.2, 37°C, recombinant catalytic C-terminal domain Bos taurus