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Literature summary extracted from

  • Adachi, N.; Oyasu, M.; Taniguchi, T.; Yamaguchi, Y.; Takenaka, R.; Shirai, Y.; Saito, N.
    Immunocytochemical localization of a neuron-specific diacylglycerol kinase beta and gamma in the developing rat brain (2005), Mol. Brain Res., 139, 288-299.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 GFP-tagged DGKh and DGKg co-expressed in human neuroblastoma cells SH-SY5Y, expression of GST-fusion isozyme DGKalpha and DGKgamma in Escherichia coli strain DH5alpha, expression of GST-fusion isozyme DGKbeta in Spodoptera frugiperda Sf9 cells via baculovirus infection system, expression of GST-fusion isozymes DGKalpha, DGKbeta, and DGKgamma in COS-7 cells Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 cytosol isozyme DGKgamma in hippocampal pyriamidal cells and proximal dendrites Rattus norvegicus 5829
-
2.7.1.107 additional information no isozyme DGKgamma in the nucleus, subcellular localization of isozymes in neurons Rattus norvegicus
-
-
2.7.1.107 plasma membrane isozyme DGKbeta predominantly, in hippocampal pyriamidal neurons including perikarya and the peripheral dendrites, dendrit cell body Rattus norvegicus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Ca2+ dependent on, isozymes DGKalpha, DGKbeta, and DGKgamma Rattus norvegicus
2.7.1.107 Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol Rattus norvegicus termination of diacylglycerol signaling, isozymes DGKalpha, DGKbeta, and DGKgamma play a pivotal role in development and metabolism of brain ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Rattus norvegicus
-
 neuron-specific isozymes DGKbeta and DGKgamma
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 recombinant GST-fusion isozymes DGKalpha, DGKgamma, and DGKbeta from Escherichia coli, and Sf9 cells, respectively, and from COS-7 cells, by glutathione affinity chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.107 brain post-natal, developing, neuron-specific isozymes DGKbeta and DGKgamma, and isozyme DGKalpha, isozymes alpha and gamma appear at birth and then decline, while isozyme beta appears about 14 days after birth and reaches a maximum at day 28, overview Rattus norvegicus
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2.7.1.107 hippocampus pyramidal cells, isozymes DGKbeta and DGKgamma Rattus norvegicus
-
2.7.1.107 medial geniculate nucleus isozyme DGKgamma, day 3-14 after birth Rattus norvegicus
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2.7.1.107 neuron neuron-specific isozymes DGKbeta and DGKgamma having a spatio-temporally different function in the neuron Rattus norvegicus
-
2.7.1.107 Purkinje cell delay of isozyme DGKgamma Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacylglycerol
-
 Rattus norvegicus ADP + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
2.7.1.107 ATP + 1,2-diacylglycerol termination of diacylglycerol signaling, isozymes DGKalpha, DGKbeta, and DGKgamma play a pivotal role in development and metabolism of brain Rattus norvegicus ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.107 ? x * 88000-90000, isozymes DGKalpha, DGKbeta, and DGKgamma, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.107 DGK
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.107 ATP
-
 Rattus norvegicus