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Literature summary extracted from

  • Muerhoff, A.S.; Dawson, G.J.; Desai, S.M.
    A non-isotopic method for the determination of activity of the thermostable NAD-dependent DNA ligase from Thermus thermophilus HB8 (2004), J. Virol. Methods, 119, 171-176.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Thermus thermophilus HB8
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.5.1.2 additional information
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 non-isotopic method for the determination of activity of the thermostable NAD-dependent DNA ligase from Thermus thermophilus HB8. The assay uses the cohesive ends of restriction endonuclease digested lambda-DNA as substrate. Separation of ligated from unligated cohesive end-containing fragments via agarose gel electrophoresis, followed by digital image analysis employing a normalization procedure to correct for artifacts of gel electrophoresis, allows the accurate determination of unit ligase activity Thermus thermophilus HB8

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m cohesive ends of restriction endonuclease digested lambda-DNA Thermus thermophilus HB8 AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
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