Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Varkondi, E.; Schaefer, E.; Boekoenyi, G.; Gyoekeres, T.; Oerfi, L.; Petak, I.; Pap, A.; Szokoloczi, O.; Keri, G.; Schwab, R.
    Comparison of ELISA-based tyrosine kinase assays for screening EGFR inhibitors (2005), J. Recept. Signal Transduct. Res., 25, 45-56.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.10.1 expression of EGFR-TK in Spodoptera frugiperda Sf9 cells using the baculovirus infection system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.10.1 gefitinib specific inhibition of EGFR-TK Homo sapiens
2.7.10.1 PD153035 specific inhibition of EGFR-TK, IC50 is 0.00329 mM with 1 U of purified EGFR-TK from A431 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.10.1 additional information
-
 additional information kinetics of wild-type EGFR-TK from A431 cells and recombinant EGFR-TK from Sf9 cells Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.10.1 ATP + a [protein]-L-tyrosine Homo sapiens
-
 ADP + a [protein]-L-tyrosine phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.10.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.10.1 EGFR-TK from A431 cells by affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.10.1 A-431 cell
-
 Homo sapiens
-
2.7.10.1 brain tumor cell overexpression of EGFR-TK Homo sapiens
-
2.7.10.1 breast cancer cell overexpression of EGFR-TK Homo sapiens
-
2.7.10.1 colonic cancer cell overexpression of EGFR-TK Homo sapiens
-
2.7.10.1 lung cancer cell overexpression of EGFR-TK Homo sapiens
-
2.7.10.1 ovarian cancer cell overexpression of EGFR-TK Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.10.1 additional information
-
 development of a specific ELISA-based phosphotyrosine detection Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.10.1 ATP + a [protein]-L-tyrosine
-
 Homo sapiens ADP + a [protein]-L-tyrosine phosphate
-
 ?
2.7.10.1 ATP + [poly-(Glu,Tyr)1:4] synthetic substrate Homo sapiens ADP + [poly-(Glu,Tyr)1:4]-tyrosine phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.10.1 EGFR tyrosine kinase
-
 Homo sapiens
2.7.10.1 EGFR-TK
-
 Homo sapiens
2.7.10.1 epidermal growth factor receptor tyrosine kinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.10.1 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.10.1 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.10.1 ATP
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.10.1 0.00329
-
 specific inhibition of EGFR-TK, IC50 is 0.00329 mM with 1 U of purified EGFR-TK from A431 cells Homo sapiens PD153035