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Literature summary extracted from

  • Hamada, K.; Nishida, T.; Yamauchi, K.; Fukushima, K.; Kondo, R.; Tsutsumi, Y.
    4-Coumarate:coenzyme A ligase in black locust (Robinia pseudoacacia) catalyses the conversion of sinapate to sinapoyl-CoA (2004), J. Plant Res., 117, 303-310.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.12 additional information the sinapate-converting activity is not inhibited by other cinnamate derivatives, such as p-coumarate, caffeate or ferulate Robinia pseudoacacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.12 62000
-
 x * 62000, SDS-PAGE Robinia pseudoacacia

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Robinia pseudoacacia
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 shoot
-
 Robinia pseudoacacia
-
6.2.1.12 xylem 4CL2 is the major isoform in developing xylem extract, 4CL3 is a very minor one Robinia pseudoacacia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA isoenzyme 4CL1 exhibits very high activity towards p-coumarate, isoenzymes 4CL2 and 4CL3 are active Robinia pseudoacacia AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 5-hydroxyferulate + CoA isoenzyme 4CL2, weak activity, isoenzyme 4CL1 and 4CL3 show no activity Robinia pseudoacacia AMP + diphosphate + 5-hydroxyferuloyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA isoenzyme 4CL1, 4CL2 and 4CL3 show activity Robinia pseudoacacia AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA isoenzyme 4CL2 and 4CL3 are active, no activity with isoenzyme 4CL1 Robinia pseudoacacia AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + sinapate + CoA isoenzymes 4CL2 and 4CL3 are active, isoenzyme 4CL1 is inactive Robinia pseudoacacia AMP + diphosphate + sinapoyl-CoA
-
 ?
6.2.1.12 additional information the isoform 4CL1 has a strong substrate preference for p-coumarate, but lacked the activity for ferulate and sinapate. The other hand, 4CL2 and 4CL3 display activity towards sinapate and also possess high activity towards caffeate as well as p-coumarate Robinia pseudoacacia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.12 ? x * 62000, SDS-PAGE Robinia pseudoacacia

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-coumarate:coenzyme A (CoA) ligase
-
 Robinia pseudoacacia
6.2.1.12 4CL
-
 Robinia pseudoacacia