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Literature summary extracted from

  • Deng, J.; Schnaufer, A.; Salavati, R.; Stuart, K.D.; Hol, W.G.
    High resolution crystal structure of a key editosome enzyme from Trypanosoma brucei:RNA editing ligase 1 (2004), J. Mol. Biol., 343, 601-613.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.3 expression in Escherichia coli BL21goldDE3 Trypanosoma brucei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.5.1.3 crystal structure of the catalytic domain of TbREL1 at 1.2 A resolution, in complex with ATP and magnesium.The magnesium ion interacts with the beta and gamma-phosphate groups and is almost perfectly octahedrally coordinated by six phosphate and water oxygen atoms. Sitting-drop, vapor-diffusion with 100 mM HEPES, 100 mM Mg2+ and 35% (w/v) PEG3350 at pH 7.0. The crystal belongs to space group P2(1), with cell dimensions of a = 44.9 A, b = 58.6 A, c = 53.0 A and beta = 100.2°, and one molecule in the asymmetric unit Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.3 Mg2+ magnesium ion interacts with the beta and gamma-phosphate groups and is almost perfectly octahedrally coordinated by six phosphate and water oxygen atoms Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m Trypanosoma brucei the enzyme is essential for survival of both insect and bloodstream forms of the parasite AMP + diphosphate + (ribonucleotide)n+m
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 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.3 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.3 recombinant Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m
-
 Trypanosoma brucei AMP + diphosphate + (ribonucleotide)n+m
-
 ?
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m the enzyme is essential for survival of both insect and bloodstream forms of the parasite Trypanosoma brucei AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.3 RNA editing ligase 1
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 Trypanosoma brucei
6.5.1.3 TbREL1
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 Trypanosoma brucei