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Literature summary extracted from

  • Wadgaonkar, R.; Dudek, S.M.; Zaiman, A.L.; Linz-McGillem, L.; Verin, A.D.; Nurmukhambetova, S.; Romer, L.H.; Garcia, J.G.
    Intracellular interaction of myosin light chain kinase with macrophage migration inhibition factor (MIF) in endothelium (2005), J. Cell. Biochem., 95, 849-858.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.18 additional information the endothelial isozyme EC MLCK contains a unique N-terminal sequence which is a target for upregulation by signaling cascade components such as p60src Homo sapiens
2.7.11.18 thrombin stimulates endothelial isozyme activity in endothelium, and increases EC MLCK interaction with macrophage migration inhibition factor MIF Homo sapiens
2.7.11.18 TNFalpha stimulates endothelial isozyme activity in endothelium, and increases EC MLCK interaction with macrophage migration inhibition factor MIF Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.18 expression of epitope-tagged endothelial isozyme in endothelial cells, expression of GST-fusion isozyme EC MLCK in bacteria, expression of endothelial iszyme EC MLCK and macrophage migration inhibition factor MIF in the yeast two-hybrid system, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + [myosin light chain] Homo sapiens the endothelial isozyme is a mutifunctional contractile effector involved in vascular barrier regulation, leukocyte diapedesis, apoptosis, and angiogenesis ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 additional information Homo sapiens the enzyme interacts with macrophage migration inhibition factor MIF in endothelium which may be important for the regulation of both non-muscle cytoskeletal dynamics as well as pathobiological vascular events involving the enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Homo sapiens
-
 endothelial isozyme EC MLCK exists in different splice variants
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.18 recombinant GST-fusion isozyme EC MLCK by affinity chromatgraphy Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 endothelium endothelial isozyme EC MLCK Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + [myosin light chain] the endothelial isozyme is a mutifunctional contractile effector involved in vascular barrier regulation, leukocyte diapedesis, apoptosis, and angiogenesis Homo sapiens ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 ATP + [myosin light chain] activated phosphorylated enzyme Homo sapiens ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 additional information the enzyme interacts with macrophage migration inhibition factor MIF in endothelium which may be important for the regulation of both non-muscle cytoskeletal dynamics as well as pathobiological vascular events involving the enzyme Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 More the endothelial isozyme EC MLCK contains a unique N-terminal sequence which is a target for upregulation by signaling cascade components such as p60src, EC MLCK splice variants doamin structure Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 EC MLCK
-
 Homo sapiens
2.7.11.18 endothelial cell myosin light chain kinase
-
 Homo sapiens
2.7.11.18 myosin light chain kinase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 ATP
-
 Homo sapiens
2.7.11.18 Calmodulin
-
 Homo sapiens