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Literature summary extracted from

  • Wadgaonkar, R.; Linz-McGillem, L.; Zaiman, A.L.; Garcia, J.G.
    Endothelial cell myosin light chain kinase (MLCK) regulates TNFalpha-induced NFkappaB activity (2005), J. Cell. Biochem., 94, 351-364.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.18 expression of calmodulin-independent mutant MLCK1745, lacking the C-terminal part including the autoinhibitory domain, and the myosin- and the calmodulin-binding sites, and of MLVKATPdel mutant with a defective ATP-binding site in bovine pulmonary artery endothelial cells BPAEC as V5-epitope-tagged proteins Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.18 additional information construction of an enzyme mutant MLCK1745 which lacks the C-terminal amino acids including the autoinhibitory domain, and the myosin- and the calmodulin-binding sites, the MLCK1745 mutant increases the TNFalpha response to upregulate NFkappaB activity in transfected endothelial cells, MLCKATPdel mutant enzyme with defective ATP binding site inhibits TNFalpha-induced NFkappaB-activation activity in transfected endothelial cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.18 ML-7 specific inhibitor Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + [myosin light chain] Homo sapiens
-
 ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 additional information Homo sapiens the enzyme plays an important role in TNFalpha-dependent induction of NFkappaB activity, mechanism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.18 recombinant wild-type and mutant enzymes from transfected bovine endothelial cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 endothelial cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + [myosin light chain]
-
 Homo sapiens ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 ATP + [myosin light chain] activated phosphorylated enzyme Homo sapiens ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 additional information the enzyme plays an important role in TNFalpha-dependent induction of NFkappaB activity, mechanism Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 EC MLCK
-
 Homo sapiens
2.7.11.18 endothelial cell myosin light chain kinase
-
 Homo sapiens
2.7.11.18 MLCK
-
 Homo sapiens
2.7.11.18 myosin light chain kinase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 ATP
-
 Homo sapiens
2.7.11.18 Calmodulin
-
 Homo sapiens