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Literature summary extracted from

  • Jordens, J.; Janssens, V.; Longin, S.; Stevens, I.; Martens, E.; Bultynck, G.; Engelborghs, Y.; Lescrinier, E.; Waelkens, E.; Goris, J.; Van Hoof, C.
    The PP2A phosphatase activator (PTPA) is a novel peptidyl-prolyl cis/trans isomerase (2005), J. Biol. Chem., 281, 6349-6357.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.2.1.8 ATP stimulation Homo sapiens
5.2.1.8 ATP stimulation Saccharomyces cerevisiae
5.2.1.8 ATP at least 3fold stimulation, excess of ATP over Mg2+ is inhibitory Saccharomyces cerevisiae
5.2.1.8 ATP at least 3fold stimulation, excess of ATP over Mg2+ is inhibitory Oryctolagus cuniculus

Protein Variants

EC Number Protein Variants Comment Organism
5.2.1.8 D205G essential for petidyl-prolyl cis/trans isomerase activity and activation of PP2A phosphatase Saccharomyces cerevisiae
5.2.1.8 D205G D205 is required for both peptidylprolyl isomerase activity and protein phosphatase 2A activation Saccharomyces cerevisiae
5.2.1.8 DELTA208-213 almost no enzymic activity, 400fold less activity in the activation reaction of PP2A phosphatase Oryctolagus cuniculus
5.2.1.8 DELTA208-213 dramatic reduction of peptidylprolyl isomerase activity and 400fold reduction of protein phosphatase 2A activation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.2.1.8 ATP at least 3fold stimulation, excess of ATP over Mg2+ is inhibitory Oryctolagus cuniculus
5.2.1.8 ATP at least 3fold stimulation, excess of ATP over Mg2+ is inhibitory Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.2.1.8 Mg2+ stimulation Homo sapiens
5.2.1.8 Mg2+ stimulation Saccharomyces cerevisiae
5.2.1.8 Mg2+ at least 3fold stimulation Saccharomyces cerevisiae
5.2.1.8 Mg2+ at least 3fold stimulation Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.2.1.8 PP2A phosphatase with cis-P190 Saccharomyces cerevisiae
-
 PP2A phosphatase with trans-P190
-
 ?
5.2.1.8 PP2A phosphatase with cis-P190 Oryctolagus cuniculus
-
 PP2A phosphatase with trans-P190
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.2.1.8 Homo sapiens
-
-
-
5.2.1.8 Oryctolagus cuniculus
-
 recombinant wild-type enzyme and a His-tagged version
-
5.2.1.8 Saccharomyces cerevisiae
-
 isoforms Ypa1, Ypa2
-
5.2.1.8 Saccharomyces cerevisiae
-
 isoform Ypa1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.2.1.8 (trans)-Pro190 of protein phosphatase 2A
-
 Homo sapiens (cis)-Pro190 of protein phosphatase 2A
-
 ?
5.2.1.8 (trans)-Pro190 of protein phosphatase 2A
-
 Saccharomyces cerevisiae (cis)-Pro190 of protein phosphatase 2A
-
 ?
5.2.1.8 PP2A phosphatase with cis-P190
-
 Saccharomyces cerevisiae PP2A phosphatase with trans-P190
-
 ?
5.2.1.8 PP2A phosphatase with cis-P190
-
 Oryctolagus cuniculus PP2A phosphatase with trans-P190
-
 ?
5.2.1.8 Suc-Ala-Ala-(trans)-Pro-Lys-p-nitroanilide
-
 Homo sapiens Suc-Ala-Ala-(cis)-Pro-Lys-p-nitroanilide
-
 ?
5.2.1.8 Suc-Ala-Ala-(trans)-Pro-Lys-p-nitroanilide
-
 Saccharomyces cerevisiae Suc-Ala-Ala-(cis)-Pro-Lys-p-nitroanilide
-
 ?
5.2.1.8 Suc-Ala-Ala-(trans)-Pro-Phe-methylcoumarylamide
-
 Homo sapiens Suc-Ala-Ala-(cis)-Pro-Phe-methylcoumarylamide
-
 ?
5.2.1.8 Suc-Ala-Ala-(trans)-Pro-Phe-methylcoumarylamide
-
 Saccharomyces cerevisiae Suc-Ala-Ala-(cis)-Pro-Phe-methylcoumarylamide
-
 ?
5.2.1.8 Suc-Ala-Ala-(trans)-Pro-Phe-p-nitroanilide
-
 Homo sapiens Suc-Ala-Ala-(cis)-Pro-Phe-p-nitroanilide
-
 ?
5.2.1.8 Suc-Ala-Ala-(trans)-Pro-Phe-p-nitroanilide
-
 Saccharomyces cerevisiae Suc-Ala-Ala-(cis)-Pro-Phe-p-nitroanilide
-
 ?
5.2.1.8 Suc-Ala-Glu-(trans)-Pro-Phe-p-nitroanilide
-
 Homo sapiens Suc-Ala-Glu-(cis)-Pro-Phe-p-nitroanilide
-
 ?
5.2.1.8 Suc-Ala-Glu-(trans)-Pro-Phe-p-nitroanilide
-
 Saccharomyces cerevisiae Suc-Ala-Glu-(cis)-Pro-Phe-p-nitroanilide
-
 ?
5.2.1.8 succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide
-
 Saccharomyces cerevisiae succinyl-Ala-Ala-(trans)-Pro-Lys + 7-amino-4-methylcoumarin
-
 ?
5.2.1.8 succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide
-
 Oryctolagus cuniculus succinyl-Ala-Ala-(trans)-Pro-Lys + 7-amino-4-methylcoumarin
-
 ?
5.2.1.8 succinyl-Ala-Ala-(cis)-Pro-Phe 4-methylcoumarin 7-amide
-
 Saccharomyces cerevisiae succinyl-Ala-Ala-(trans)-Pro-Phe + 7-amino-4-methylcoumarin
-
 ?
5.2.1.8 succinyl-Ala-Ala-(cis)-Pro-Phe 4-methylcoumarin 7-amide
-
 Oryctolagus cuniculus succinyl-Ala-Ala-(trans)-Pro-Phe + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.2.1.8 PP2A phosphatase activator
-
 Oryctolagus cuniculus
5.2.1.8 protein phosphatase 2A phosphatase activator
-
 Homo sapiens
5.2.1.8 PtpA
-
 Homo sapiens
5.2.1.8 PtpA
-
 Oryctolagus cuniculus
5.2.1.8 Ypa
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.2.1.8 0.006
-
 succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide 20°C, pH 7.4, mutant DELTA208-213 Oryctolagus cuniculus
5.2.1.8 0.008
-
 succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide 20°C, pH 7.4, mutant DELTA208-213, presence of 1 mM MgATP Oryctolagus cuniculus
5.2.1.8 0.011
-
 succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide 20°C, pH 7.4, mutant D205G of Ypa1 Saccharomyces cerevisiae
5.2.1.8 0.016
-
 succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide 20°C, pH 7.4, mutant D205G of Ypa1, presence of 1 mM MgATP Saccharomyces cerevisiae
5.2.1.8 0.02
-
 succinyl-Ala-Ala-(cis)-Pro-Phe 4-methylcoumarin 7-amide 20°C, pH 7.4, wild-type Ypa1 Saccharomyces cerevisiae
5.2.1.8 0.03
-
 succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide 20°C, pH 7.4, wild-type Oryctolagus cuniculus
5.2.1.8 0.03
-
 succinyl-Ala-Ala-(cis)-Pro-Phe 4-methylcoumarin 7-amide 20°C, pH 7.4, wild-type Oryctolagus cuniculus
5.2.1.8 0.03
-
 succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide 20°C, pH 7.4, wild-type Ypa2 Saccharomyces cerevisiae
5.2.1.8 0.05
-
 succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide 20°C, pH 7.4, wild-type Ypa2, presence of 1 mM MgATP Saccharomyces cerevisiae
5.2.1.8 0.07
-
 succinyl-Ala-Ala-(cis)-Pro-Phe 4-methylcoumarin 7-amide 20°C, pH 7.4, wild-type Ypa1, presence of 1 mM MgATP Saccharomyces cerevisiae
5.2.1.8 0.1
-
 succinyl-Ala-Ala-(cis)-Pro-Lys-4-methylcoumarin-7-amide 20°C, pH 7.4, wild-type, presence of 1 mM MgATP Oryctolagus cuniculus
5.2.1.8 0.19
-
 succinyl-Ala-Ala-(cis)-Pro-Phe 4-methylcoumarin 7-amide 20°C, pH 7.4, wild-type, presence of 1 mM MgATP Oryctolagus cuniculus