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Literature summary extracted from

  • Iyer, G.H.; Moore, M.J.; Taylor, S.S.
    Consequences of lysine 72 mutation on the phosphorylation and activation state of cAMP-dependent kinase (2005), J. Biol. Chem., 280, 8800-8807.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.11 cAMP dependent on Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.11 expression of PKA catalytic subunit Calpha as His-tagged protein in Escherichia coli strain BL21(DE3) and of HA-tagged wild-type and mutant PKA Calpha in COS cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.11 K72A site-directed mutagenesis, inactive unphosphorylated mutant Mus musculus
2.7.11.11 K72H site-directed mutagenesis, inactive unphosphorylated mutant Mus musculus
2.7.11.11 K72M site-directed mutagenesis, inactive unphosphorylated mutant Mus musculus
2.7.11.11 K72R site-directed mutagenesis, inactive unphosphorylated mutant Mus musculus
2.7.11.11 S338A site-directed mutagenesis, mutant is phosphorylated at T197, reduced activity compared to the wild-type enzyme Mus musculus
2.7.11.11 T197A site-directed mutagenesis, mutant is not phosphorylated, inactive mutant Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.11 H-89 inhibits autophosphorylation of the catalytic subunit C, reversible Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.11 Mg2+
-
 Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.11 phosphoprotein phosphorylation at T197 by exogenous kinase PDK-I, the enzyme performs autophosphorylation at K72, S338 and T197, the latter is phosphorylated first and required for activity, phosphorylation of the other 2 positions take place only if T197 is already phosphorylated, mechanism Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 ATP + Kemptide LRRASLG peptides substrate Mus musculus ADP + phosphorylated Kemptide LRRA-phosphoserine-LG ?
2.7.11.11 additional information the enzyme performs autophosphorylation at K72, S338, and T197 Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.11 30
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP
-
 Mus musculus