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Literature summary extracted from

  • Bonner, E.R.; Cahoon, R.E.; Knapke, S.M.; Jez, J.M.
    Molecular basis of cysteine biosynthesis in plants: Structural and functional analysis of O-acetylserine sulfhydrylase from Arabidopsis thaliana (2005), J. Biol. Chem., 280, 38803-38813.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.47 native protein and mutant K46A with pyridoxal 5’-phosphate and methionine covalently linked as an external aldimine Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.47 H157N comparable to wild-type Arabidopsis thaliana
2.5.1.47 H157Q reduced kcat-value, reduced Km-value Arabidopsis thaliana
2.5.1.47 K46A no enzymic activity, crystallization data Arabidopsis thaliana
2.5.1.47 N77A reduced kcat-value Arabidopsis thaliana
2.5.1.47 N77D drastically reduced kcat-value Arabidopsis thaliana
2.5.1.47 Q147A drastically reduced kcat-value, increased Km-value Arabidopsis thaliana
2.5.1.47 Q147E drastically reduced kcat-value, increased Km-value Arabidopsis thaliana
2.5.1.47 S269A reduced kcat-value, reduced Km-value Arabidopsis thaliana
2.5.1.47 S269T reduced kcat-value, reduced Km-value Arabidopsis thaliana
2.5.1.47 S75A drastically reduced kcat-value Arabidopsis thaliana
2.5.1.47 S75N drastically reduced kcat-value Arabidopsis thaliana
2.5.1.47 S75T drastically reduced kcat-value Arabidopsis thaliana
2.5.1.47 T182A reduced kcat-value Arabidopsis thaliana
2.5.1.47 T182S comparable to wild-type Arabidopsis thaliana
2.5.1.47 T185A drastically reduced kcat-value Arabidopsis thaliana
2.5.1.47 T185S drastically reduced kcat-value Arabidopsis thaliana
2.5.1.47 T74A drastically reduced kcat-value Arabidopsis thaliana
2.5.1.47 T74S reduced kcat-value Arabidopsis thaliana
2.5.1.47 T78A reduced kcat-value, reduced Km-value Arabidopsis thaliana
2.5.1.47 T78S reduced kcat-value, reduced Km-value Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.18
-
 Sulfide mutant N77D, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.21
-
 Sulfide mutant S75T, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.22
-
 Sulfide wild-type, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.22
-
 Sulfide mutant T182S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.22
-
 Sulfide mutant T185A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.23
-
 Sulfide mutant N77A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.27
-
 Sulfide mutant Q147S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.3
-
 Sulfide mutant H157Q, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.3
-
 Sulfide mutant T78S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.36
-
 Sulfide mutant T185S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.39
-
 Sulfide mutant T182A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.4
-
 Sulfide mutant H157N, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.44
-
 Sulfide mutant T78A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.54
-
 Sulfide mutant S269A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.57
-
 O3-acetyl-L-serine mutant S269A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.68
-
 O3-acetyl-L-serine mutant T78A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.82
-
 O3-acetyl-L-serine mutant T78S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.91
-
 O3-acetyl-L-serine mutant H157Q, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.94
-
 O3-acetyl-L-serine mutant H157N, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.96
-
 O3-acetyl-L-serine mutant S269T, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.2
-
 O3-acetyl-L-serine mutant S75T, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.2
-
 O3-acetyl-L-serine mutant T182A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.4
-
 O3-acetyl-L-serine wild-type, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.5
-
 O3-acetyl-L-serine mutant T185A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.5
-
 O3-acetyl-L-serine mutant T74S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.6
-
 Sulfide mutant S269T, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.6
-
 O3-acetyl-L-serine mutant S75A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.6
-
 O3-acetyl-L-serine mutant T182S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.6
-
 O3-acetyl-L-serine mutant T185S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.6
-
 O3-acetyl-L-serine mutant T74A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.7
-
 Sulfide mutant Q147A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 2
-
 O3-acetyl-L-serine mutant S75N, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 3.2
-
 Sulfide mutant T74S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 3.8
-
 O3-acetyl-L-serine mutant Q147S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 3.9
-
 Sulfide mutant T74A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 4.4
-
 O3-acetyl-L-serine mutant Q147A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 4.6
-
 Sulfide mutant S75A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 4.7
-
 O3-acetyl-L-serine mutant N77A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 5.7
-
 Sulfide mutant S75N, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 10.2
-
 O3-acetyl-L-serine mutant N77D, pH 7.0, 25°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O3-acetyl-L-serine + hydrogensulfide
-
 Arabidopsis thaliana L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer crystallization data Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.47 0.08
-
 O3-acetyl-L-serine mutant T74A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.09
-
 O3-acetyl-L-serine mutant S75N, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.19
-
 O3-acetyl-L-serine mutant Q147S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.22
-
 Sulfide mutant Q147S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.34
-
 O3-acetyl-L-serine mutant N77D, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.42
-
 Sulfide mutant T74A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 0.73
-
 Sulfide mutant N77D, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.07
-
 O3-acetyl-L-serine mutant T185A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.44
-
 Sulfide mutant T185A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1.45
-
 Sulfide mutant S75N, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 3.11
-
 O3-acetyl-L-serine mutant T185S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 4.02
-
 O3-acetyl-L-serine mutant Q147A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 5.57
-
 Sulfide mutant T185S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 7.16
-
 O3-acetyl-L-serine mutant S75A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 12.5
-
 Sulfide mutant Q147A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 20.2
-
 Sulfide mutant S75A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 29.5
-
 Sulfide mutant S269A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 34.3
-
 O3-acetyl-L-serine mutant S269A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 51.5
-
 O3-acetyl-L-serine mutant S75T, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 82.7
-
 Sulfide mutant S75T, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 155
-
 Sulfide mutant N77A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 370
-
 Sulfide mutant T78S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 403
-
 O3-acetyl-L-serine mutant N77A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 456
-
 O3-acetyl-L-serine mutant T74S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 537
-
 Sulfide mutant T78A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 554
-
 O3-acetyl-L-serine mutant T78A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 572
-
 O3-acetyl-L-serine mutant S269T, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 775
-
 O3-acetyl-L-serine mutant T78S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 889
-
 O3-acetyl-L-serine mutant T182A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 929
-
 Sulfide mutant S269T, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1055
-
 O3-acetyl-L-serine mutant H157Q, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1520
-
 O3-acetyl-L-serine mutant H157N, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1600
-
 Sulfide mutant T182A, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1660
-
 O3-acetyl-L-serine mutant T182S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1690
-
 Sulfide mutant T182S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1700
-
 Sulfide mutant T74S, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1780
-
 O3-acetyl-L-serine wild-type, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1780
-
 Sulfide mutant H157Q, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 1990
-
 Sulfide mutant H157N, pH 7.0, 25°C Arabidopsis thaliana
2.5.1.47 2170
-
 Sulfide wild-type, pH 7.0, 25°C Arabidopsis thaliana