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Literature summary extracted from

  • Schertzer, J.W.; Bhavsar, A.P.; Brown, E.D.
    Two conserved histidine residues are critical to the function of the TagF-like family of enzymes (2005), J. Biol. Chem., 280, 36683-36690.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.12 D630A the ratio of turnover number to KM-value is 3.3fold lower than the wild-type ratio Bacillus subtilis
2.7.8.12 D645A the ratio of turnover number to KM-value is 3.5fold lower than the wild-type ratio Bacillus subtilis
2.7.8.12 E604A the ratio of turnover number to KM-value is 1.3fold lower than the wild-type ratio Bacillus subtilis
2.7.8.12 E639A the ratio of turnover number to KM-value is 3.9fold lower than the wild-type ratio Bacillus subtilis
2.7.8.12 H474A the ratio of turnover number to KM-value is 4900fold lower than the wild-type ratio Bacillus subtilis
2.7.8.12 H612A the ratio of turnover number to KM-value is more than 4900fold lower than the wild-type ratio Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.12 0.23
-
 CDP-glycerol wild-type enzyme Bacillus subtilis
2.7.8.12 0.23
-
 CDP-glycerol mutant enzyme E604A Bacillus subtilis
2.7.8.12 0.39
-
 CDP-glycerol mutant enzyme D630A Bacillus subtilis
2.7.8.12 0.7
-
 CDP-glycerol mutant enzyme E639A Bacillus subtilis
2.7.8.12 0.75
-
 CDP-glycerol mutant enzyme D645A Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.12 CDP-glycerol + (glycerophosphate)n Bacillus subtilis the enzyme is responsible for the synthesis of wall teichoic acid CMP + (glycerophosphate)n+1
-
 ?
2.7.8.12 CDP-glycerol + (glycerophosphate)n Bacillus subtilis 168 the enzyme is responsible for the synthesis of wall teichoic acid CMP + (glycerophosphate)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.12 Bacillus subtilis
-
-
-
2.7.8.12 Bacillus subtilis 168
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.12
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
2.7.8.12 -80°C, the carboxy-terminal His6-tagged TagF protein is stable for several months Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.12 CDP-glycerol + (glycerophosphate)n the enzyme is responsible for the synthesis of wall teichoic acid Bacillus subtilis CMP + (glycerophosphate)n+1
-
 ?
2.7.8.12 CDP-glycerol + (glycerophosphate)n the enzyme is responsible for the synthesis of wall teichoic acid Bacillus subtilis 168 CMP + (glycerophosphate)n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.12 poly(glycerol phosphate)polymerase
-
 Bacillus subtilis
2.7.8.12 TagF protein
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.8.12 0.12
-
 CDP-glycerol mutant enzyme D630A Bacillus subtilis
2.7.8.12 0.167
-
 CDP-glycerol mutant enzyme E604A Bacillus subtilis
2.7.8.12 0.167
-
 CDP-glycerol mutant enzyme E639A Bacillus subtilis
2.7.8.12 0.217
-
 CDP-glycerol mutant enzyme D645A Bacillus subtilis
2.7.8.12 0.233
-
 CDP-glycerol wild-type enzyme Bacillus subtilis