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Literature summary extracted from

  • Ling, C.; Yao, Y.N.; Zheng, Y.G.; Wei, H.; Wang, L.; Wu, X.F.; Wang, E.D.
    The C-terminal appended domain of human cytosolic leucyl-tRNA synthetase is indispensable in its interaction with arginyl-tRNA synthetase in the multi-tRNA synthetase complex (2005), J. Biol. Chem., 280, 34755-34763.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 expression of hcLeuRS and DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) in a baculovirus system Homo sapiens
6.1.1.19 full-length enzyme and mutant DELTA1-72 tagged with green fluorescence protein, expression in 293 T cells, exclusively in cytosol Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.0014
-
 tRNALeu 37°C, pH 7.6, tRNALeu from calf liver, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
6.1.1.4 0.0019
-
 tRNALeu 37°C, pH 7.6, tRNALeu from calf liver, leucylation, full-length enzyme Homo sapiens
6.1.1.4 0.0058
-
 L-leucine 37°C, pH 7.6, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
6.1.1.4 0.0075
-
 L-leucine 37°C, pH 7.6, leucylation, full-length enzyme Homo sapiens
6.1.1.4 0.064
-
 L-leucine 37°C, pH 7.6, ATP-diphosphate exchange, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
6.1.1.4 0.075
-
 L-leucine 37°C, pH 7.6, ATP-diphosphate exchange, full-length enzyme Homo sapiens
6.1.1.4 0.725
-
 ATP 37°C, pH 7.6, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
6.1.1.4 0.773
-
 ATP 37°C, pH 7.6, leucylation, full-length enzyme Homo sapiens
6.1.1.4 1.308
-
 ATP 37°C, pH 7.6, ATP-diphosphate exchange, full-length enzyme Homo sapiens
6.1.1.4 1.349
-
 ATP 37°C, pH 7.6, ATP-diphosphate exchange, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
6.1.1.19 0.00005
-
 tRNAArg 37°C, pH 7.5, arginylation, full-length enzyme Homo sapiens
6.1.1.19 0.000098
-
 tRNAArg 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 Homo sapiens
6.1.1.19 0.0035
-
 L-arginine 37°C, pH 7.5, arginylation, full-length enzyme Homo sapiens
6.1.1.19 0.0048
-
 L-arginine 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 Homo sapiens
6.1.1.19 0.671
-
 ATP 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 Homo sapiens
6.1.1.19 0.91
-
 ATP 37°C, pH 7.5, arginylation, full-length enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.4 cytosol predominantly Homo sapiens 5829
-
6.1.1.19 cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 135000
-
 x * 135000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu Homo sapiens human cytosolic leucyl-tRNA synthetase is one component of a macromolecular aminoacyl-tRNA synthetase complex. The C-terminal peptide of hcLeuRS is critical for the interaction with hcArgRS and the interaction in the multi-tRNA synthetase complex AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.19 ATP + L-arginine + tRNAArg Homo sapiens the C-terminal extension of the human cytosolic leucyl-tRNA synthetase is indispensable for its interaction with the N-terminal of human cytosolic arginyl-tRNA synthetase in the macromolecular complex AMP + diphosphate + L-arginyl-tRNAArg
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Homo sapiens
-
-
-
6.1.1.19 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4 recombinant enzyme Homo sapiens
6.1.1.19
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Homo sapiens AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu human cytosolic leucyl-tRNA synthetase is one component of a macromolecular aminoacyl-tRNA synthetase complex. The C-terminal peptide of hcLeuRS is critical for the interaction with hcArgRS and the interaction in the multi-tRNA synthetase complex Homo sapiens AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.19 ATP + L-arginine + tRNAArg
-
 Homo sapiens AMP + diphosphate + L-arginyl-tRNAArg
-
 ?
6.1.1.19 ATP + L-arginine + tRNAArg the C-terminal extension of the human cytosolic leucyl-tRNA synthetase is indispensable for its interaction with the N-terminal of human cytosolic arginyl-tRNA synthetase in the macromolecular complex Homo sapiens AMP + diphosphate + L-arginyl-tRNAArg
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 ? x * 135000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 HcleuRS
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 0.028
-
 tRNALeu 37°C, pH 7.6, tRNALeu from calf liver, leucylation, full-length enzyme Homo sapiens
6.1.1.4 0.059
-
 tRNALeu 37°C, pH 7.6, tRNALeu from calf liver, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
6.1.1.4 0.27
-
 ATP 37°C, pH 7.6, leucylation, full-length enzyme Homo sapiens
6.1.1.4 0.3
-
 L-leucine 37°C, pH 7.6, leucylation, full-length enzyme Homo sapiens
6.1.1.4 0.55
-
 ATP 37°C, pH 7.6, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
6.1.1.4 0.56
-
 L-leucine 37°C, pH 7.6, leucylation, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
6.1.1.4 0.74
-
 ATP 37°C, pH 7.6, ATP-diphosphate exchange, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
6.1.1.4 0.79
-
 ATP 37°C, pH 7.6, ATP-diphosphate exchange, full-length enzyme Homo sapiens
6.1.1.4 0.81
-
 L-leucine 37°C, pH 7.6, ATP-diphosphate exchange, DELTAChcLeuRS (a C-terminal 89-amino acid truncated enzyme form) Homo sapiens
6.1.1.4 0.82
-
 L-leucine 37°C, pH 7.6, ATP-diphosphate exchange, full-length enzyme Homo sapiens
6.1.1.19 0.28
-
 ATP 37°C, pH 7.5, arginylation, full-length enzyme Homo sapiens
6.1.1.19 0.32
-
 tRNAArg 37°C, pH 7.5, arginylation, full-length enzyme Homo sapiens
6.1.1.19 0.41
-
 L-arginine 37°C, pH 7.5, arginylation, full-length enzyme Homo sapiens
6.1.1.19 0.77
-
 ATP 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 Homo sapiens
6.1.1.19 0.81
-
 tRNAArg 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 Homo sapiens
6.1.1.19 0.97
-
 L-arginine 37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72 Homo sapiens