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Literature summary extracted from

  • Mori, S.; Kawai, S.; Shi, F.; Mikami, B.; Murata, K.
    Molecular conversion of NAD Kinase to NADH kinase through single amino acid residue substitution (2005), J. Biol. Chem., 280, 24104-24112.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.86 expressed in Escherichia coli BL21(DE3)pLysS cells Mycobacterium tuberculosis
2.7.1.86 expressed in Escherichia coli BL21(DE3)pLysS cells Micrococcus flavus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.23 G183R decrease both in NAD+ kinase and NADH kinase activity Micrococcus flavus
2.7.1.23 R175E no conversion of NAD+ kinase activity to NADH kinase activity Escherichia coli
2.7.1.23 R175G shows both ATP-dependent NAD+ kinase activity and NADH kinase activity Escherichia coli
2.7.1.23 R175H shows both ATP-dependent NAD+ kinase activity and NADH kinase activity Escherichia coli
2.7.1.23 R175I no conversion of NAD+ kinase activity to NADH kinase activity Escherichia coli
2.7.1.23 R175K no conversion of NAD+ kinase activity to NADH kinase activity Escherichia coli
2.7.1.23 R175Q shows both ATP-dependent NAD+ kinase activity and NADH kinase activity Escherichia coli
2.7.1.23 R175T shows both ATP-dependent NAD+ kinase activity and NADH kinase activity Escherichia coli
2.7.1.86 G183R mutant with partially retained NADH kinase activity Micrococcus flavus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.23 0.16
-
 ATP pH 7.0, 37°C, mutant G183R Micrococcus flavus
2.7.1.23 0.2
-
 ATP pH 7.0, 37°C, mutant R175G Escherichia coli
2.7.1.23 0.21
-
 poly(P)4 pH 7.0, 37°C, mutant G183R Micrococcus flavus
2.7.1.23 0.23
-
 ATP pH 7.0, 37°C, wild-type Escherichia coli
2.7.1.23 0.23
-
 ATP pH 7.0, 37°C, wild-type Micrococcus flavus
2.7.1.23 0.26
-
 NAD+ pH 7.0, 37°C, cosubstrate poly(P)4, wild-type Micrococcus flavus
2.7.1.23 0.33
-
 poly(P)4 pH 7.0, 37°C, wild-type Micrococcus flavus
2.7.1.23 0.53
-
 NAD+ pH 7.0, 37°C, cosubstrate ATP, wild-type Micrococcus flavus
2.7.1.23 1.43
-
 NAD+ pH 7.0, 37°C, cosubstrate ATP, mutant G183R Micrococcus flavus
2.7.1.23 1.85
-
 NAD+ pH 7.0, 37°C, wild-type Escherichia coli
2.7.1.23 1.86
-
 NAD+ pH 7.0, 37°C, cosubstrate poly(P)4, mutant G183R Micrococcus flavus
2.7.1.23 2
-
 NAD+ pH 7.0, 37°C, mutant R175G Escherichia coli
2.7.1.86 0.32
-
 ATP wild type enzyme Micrococcus flavus
2.7.1.86 0.35
-
 NADH with tetrapolyphosphate as the phosphate donor, wild type enzyme Micrococcus flavus
2.7.1.86 0.45
-
 NADH with ATP as the phosphate donor, wild type enzyme Micrococcus flavus
2.7.1.86 0.47
-
 ATP mutant enzyme G183R Micrococcus flavus
2.7.1.86 0.58
-
 Tetrapolyphosphate wild type enzyme Micrococcus flavus
2.7.1.86 0.68
-
 Tetrapolyphosphate mutant enzyme G183R Micrococcus flavus
2.7.1.86 0.72
-
 NADH with tetrapolyphosphate as the phosphate donor, mutant enzyme G183R Micrococcus flavus
2.7.1.86 1.66
-
 NADH with ATP as the phosphate donor, mutant enzyme G183R Micrococcus flavus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Escherichia coli
-
-
-
2.7.1.23 Micrococcus flavus
-
-
-
2.7.1.86 Micrococcus flavus
-
-
-
2.7.1.86 Mycobacterium tuberculosis
-
-
-
2.7.1.86 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.86 Butyl-Toyopearl 650M column chromatography Mycobacterium tuberculosis
2.7.1.86 Butyl-Toyopearl 650M column chromatography Micrococcus flavus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.23 1.4
-
 pH 7.0, 37°C, substrate NAD+, cosubstrate poly(P)4, mutant G183R Micrococcus flavus
2.7.1.23 1.78
-
 pH 7.0, 37°C, substrate NAD+, cosubstrate ATP, mutant G183R Micrococcus flavus
2.7.1.23 2.33
-
 pH 7.0, 37°C, mutant R175G, substrate NAD+ Escherichia coli
2.7.1.23 2.68
-
 pH 7.0, 37°C, mutant R175, substrate ATP Escherichia coli
2.7.1.23 8.63
-
 pH 7.0, 37°C, substrate NAD+, cosubstrate poly(P)4, wild-type Micrococcus flavus
2.7.1.23 12.45
-
 pH 7.0, 37°C, wild-type, substrate ATP Escherichia coli
2.7.1.23 12.86
-
 pH 7.0, 37°C, wild-type, substrate NAD+ Escherichia coli
2.7.1.23 18.67
-
 pH 7.0, 37°C, substrate NAD+, cosubstrate ATP, wild-type Micrococcus flavus
2.7.1.86 0.22
-
 in the presence of 5 mM tetrapolyphosphate Mycobacterium tuberculosis
2.7.1.86 0.65
-
 in the presence of 5 mM ATP Mycobacterium tuberculosis
2.7.1.86 3.04
-
 in the presence of 5 mM tetrapolyphosphate Micrococcus flavus
2.7.1.86 8.66
-
 in the presence of 5 mM ATP Micrococcus flavus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 NAD+ + ATP
-
 Escherichia coli NADP+ + ADP
-
 ?
2.7.1.23 NAD+ + ATP
-
 Micrococcus flavus NADP+ + ADP
-
 ?
2.7.1.23 NAD+ + poly(P)4
-
 Micrococcus flavus NADP+ + poly(P)3
-
 ?
2.7.1.86 ATP + NADH
-
 Mycobacterium tuberculosis ADP + NADPH
-
 ?
2.7.1.86 ATP + NADH
-
 Micrococcus flavus ADP + NADPH
-
 ?
2.7.1.86 ATP + NADH
-
 Mycobacterium tuberculosis H37Rv ADP + NADPH
-
 ?
2.7.1.86 tetrapolyphosphate + NADH
-
 Mycobacterium tuberculosis tripolyphosphate + NADPH
-
 ?
2.7.1.86 tetrapolyphosphate + NADH
-
 Micrococcus flavus tripolyphosphate + NADPH
-
 ?
2.7.1.86 tetrapolyphosphate + NADH
-
 Mycobacterium tuberculosis H37Rv tripolyphosphate + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.86 homodimer
-
 Micrococcus flavus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.23 NADK
-
 Micrococcus flavus
2.7.1.23 YfjB
-
 Escherichia coli
2.7.1.86 ATP-NADH kinase
-
 Mycobacterium tuberculosis
2.7.1.86 ATP-NADH kinase
-
 Micrococcus flavus
2.7.1.86 Mfnk exhibits polyphosphate- and ATP-dependent NADH kinase activities Micrococcus flavus
2.7.1.86 Ppnk exhibits polyphosphate- and ATP-dependent NADH kinase activities Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.86 ATP
-
 Mycobacterium tuberculosis
2.7.1.86 ATP
-
 Micrococcus flavus