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Literature summary extracted from

  • Pao, C.S.; Benovic, J.L.
    Structure/function analysis of alpha2A-adrenergic receptor interaction with G protein-coupled receptor kinase 2 (2005), J. Biol. Chem., 280, 11052-11058.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.15 alpha2A-adrenergic receptor epinephrine-activated alpha2A-adrenergic receptor activates GRK2, interaction with GRK2 via the second and third intracellular loop of the receptor, determination of regions required for specific interaction utilizing recombinant GST-tagged wild-type and several mutant alpha2A ARs, residues R225, R226, R218, K320, R322, and K358 are important, overview Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.15 expression of GRK2 in Spodoptera frugiperda Sf9 cells using the baculovirus infection system Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.15 membrane
-
 Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.15 Mg2+
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.15 ATP + alpha2A-adrenergic receptor Bos taurus
-
 ADP + phosphorylated alpha2A-adrenergic receptor
-
 ?
2.7.11.15 ATP + G protein-coupled receptor Bos taurus desensitization of agonist-activated receptor ADP + phosphorylated G protein-coupled receptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.15 phosphoprotein epinephrine-activated alpha2A-adrenergic receptor activates GRK2, interaction with GRK2 via the second and third intracellular loop of the receptor, determination of regions required for specific interaction and phosphorylation activity utilizing recombinant GST-tagged wild-type and several mutant alpha2A ARs, residues R225, R226, R218, K320, R322, and K358 are important, overview Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.15 central nervous system
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + alpha2A-adrenergic receptor
-
 Bos taurus ADP + phosphorylated alpha2A-adrenergic receptor
-
 ?
2.7.11.15 ATP + alpha2A-adrenergic receptor interaction with GRK2 via the second and third intracellular loop of the receptor, determination of regions required for specific interaction and phosphorylation activity utilizing recombinant GST-tagged wild-type and several mutant alpha2A AR substrates, residues R225, R226, R218, K320, R322, and K358 are important, overview Bos taurus ADP + phosphorylated alpha2A-adrenergic receptor
-
 ?
2.7.11.15 ATP + G protein-coupled receptor
-
 Bos taurus ADP + phosphorylated G protein-coupled receptor
-
 ?
2.7.11.15 ATP + G protein-coupled receptor desensitization of agonist-activated receptor Bos taurus ADP + phosphorylated G protein-coupled receptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.15 G protein-coupled receptor kinase 2
-
 Bos taurus
2.7.11.15 GRK2
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.15 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.15 7.5
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Bos taurus