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Literature summary extracted from

  • Choi, J.M.; Park, E.Y.; Kim, J.H.; Chang, S.K.; Cho, Y.
    Probing the functional importance of the hexameric ring structure of RNase PH (2004), J. Biol. Chem., 279, 755-764.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.56 hanging-drop vapor diffusion method. The 1.9 A crystal structures of the apo and the phosphate-bound forms of RNase PH from reveal a monomeric RNase PH with an alpha/beta-fold tightly associated into a hexameric ring structure in the form of a trimer of dimers Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.56 R127A mutation has no influence on the structure Pseudomonas aeruginosa
2.7.7.56 R69S mutation has no influence on the structure Pseudomonas aeruginosa
2.7.7.56 R69S/R77S mutation leads to the dissociation of RNase PH hexamer into dimers without perturbing the overall monomeric structure Pseudomonas aeruginosa
2.7.7.56 R74S mutation leads to the dissociation of RNase PH hexamer into dimers without perturbing the overall monomeric structure Pseudomonas aeruginosa
2.7.7.56 R74S/R77S mutation leads to the dissociation of RNase PH hexamer into dimers without perturbing the overall monomeric structure Pseudomonas aeruginosa
2.7.7.56 R77S mutation has no influence on the structure Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.56 tRNAn+1 + phosphate Pseudomonas aeruginosa catalyzes the removal of nucleotides at the 3' end of the tRNA precursor, leading to the release of nucleoside diphosphate, and generates the CCA end during the maturation process tRNAn + a nucleoside diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.56 Pseudomonas aeruginosa
-
 recombinant enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.56 recombinant enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.56 tRNAn+1 + phosphate catalyzes the removal of nucleotides at the 3' end of the tRNA precursor, leading to the release of nucleoside diphosphate, and generates the CCA end during the maturation process Pseudomonas aeruginosa tRNAn + a nucleoside diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.56 More the 1.9 A crystal structures of the apo and the phosphate-bound forms of RNase PH from reveal a monomeric RNase PH with an alpha/beta-fold tightly associated into a hexameric ring structure in the form of a trimer of dimers Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.56 45
-
 Tm-value for mutant enzymes R69S/R77S, R74S/R77S and R74S Pseudomonas aeruginosa
2.7.7.56 52
-
 Tm-value for wild-type enzyme and mutant enzyme R127A Pseudomonas aeruginosa