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The NAD biosynthesis pathway mediated by nicotinamide phosphoribosyltransferase regulates Sir2 activity in mammalian cells

Revollo, J.R.; Grimm, A.A.; Imai, S.; J. Biol. Chem. 279, 50754-50763 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.4.2.12
DNA sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme and of enzyme fused to Sir2 in Escherichia coli strain BL21(DE3)
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.92
-
nicotinamide
pH 7.4, 37°C, recombinant enzyme
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Mus musculus
key enzyme in the regulation of NAD+ biosynthesis, regulates the silent regulator protein 2, i.e. Sir2, activity, the enzyme has a metabolic regulatory function by up- and downregulation of proteins, overview
nicotinamide D-ribonucleotide + diphosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.12
Mus musculus
Q99KQ4
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.12
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.12
fibroblast
enzyme and Sir2 expression levels
Mus musculus
-
2.4.2.12
liver
-
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.12
additional information
-
-
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
additional information
pathway reconstitution in vitro using recombinant enzymes, overview
662306
Mus musculus
?
-
-
-
-
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
662306
Mus musculus
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
key enzyme in the regulation of NAD+ biosynthesis, regulates the silent regulator protein 2, i.e. Sir2, activity, the enzyme has a metabolic regulatory function by up- and downregulation of proteins, overview
662306
Mus musculus
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.12
37
-
assay at
Mus musculus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.02
-
nicotinamide
pH 7.4, 37°C, recombinant enzyme
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.12
7.4
-
assay at
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.12
DNA sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme and of enzyme fused to Sir2 in Escherichia coli strain BL21(DE3)
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.92
-
nicotinamide
pH 7.4, 37°C, recombinant enzyme
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Mus musculus
key enzyme in the regulation of NAD+ biosynthesis, regulates the silent regulator protein 2, i.e. Sir2, activity, the enzyme has a metabolic regulatory function by up- and downregulation of proteins, overview
nicotinamide D-ribonucleotide + diphosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.12
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.12
fibroblast
enzyme and Sir2 expression levels
Mus musculus
-
2.4.2.12
liver
-
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.12
additional information
-
-
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
additional information
pathway reconstitution in vitro using recombinant enzymes, overview
662306
Mus musculus
?
-
-
-
-
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
662306
Mus musculus
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
key enzyme in the regulation of NAD+ biosynthesis, regulates the silent regulator protein 2, i.e. Sir2, activity, the enzyme has a metabolic regulatory function by up- and downregulation of proteins, overview
662306
Mus musculus
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.12
37
-
assay at
Mus musculus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.02
-
nicotinamide
pH 7.4, 37°C, recombinant enzyme
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.12
7.4
-
assay at
Mus musculus