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Literature summary extracted from

  • Schulz, J.M.; Watson, A.L.; Sanders, R.; Ross, K.L.; Thoden, J.B.; Holden, H.M.; Fridovich-Keil, J.L.
    Determinants of function and substrate specificity in human UDP-galactose 4'-epimerase (2004), J. Biol. Chem., 279, 32796-32803.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.2 mutant enzymes S132A, Y157F, S132A/Y157F and C307Y are expressed in a null-background strain of Saccharomyces cerevisiae. S132a/Y157F and C307Y are also overexpressed in Pichia pastoris Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.2 C307Y normal activity with respect to UDP-galactose, complete loss of activity with respect to UDP-N-acetylgalactosamine Homo sapiens
5.1.3.2 S132A complete loss of activity with respect to interconversion of UDP-glucose and UDP-galactose and of UDP-GalNAc and UDP-GlcNAc Homo sapiens
5.1.3.2 S132A/Y157F complete loss of activity with respect to interconversion of UDP-glucose and UDP-galactose and of UDP-GalNAc and UDP-GlcNAc Homo sapiens
5.1.3.2 Y157F complete loss of activity with respect to interconversion of UDP-glucose and UDP-galactose and of UDP-GalNAc and UDP-GlcNAc Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.2 UDP-galactose Homo sapiens final step of the Leloir pathway UDP-glucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.2 recombinant wild-type and mutant enzymes S132A, Y157F, S132A/Y157F and C307Y Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.2 UDP-galactose
-
 Homo sapiens UDP-glucose
-
 r
5.1.3.2 UDP-galactose final step of the Leloir pathway Homo sapiens UDP-glucose
-
 r
5.1.3.2 UDP-GalNAc
-
 Homo sapiens UDP-GlcNAc
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.2 UDP-galactose 4'-epimerase
-
 Homo sapiens