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Literature summary extracted from

  • Singh, B.; Schwartz, N.B.
    Identification and functional characterization of the novel BM-motif in the murine phosphoadenosine phosphosulfate (PAPS) synthetase (2003), J. Biol. Chem., 278, 71-75.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.25 expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.25 D87A almost complete loss of activity Mus musculus
2.7.1.25 D87E 80% loss of activity Mus musculus
2.7.1.25 D87R almost complete loss of activity Mus musculus
2.7.1.25 D89A almost complete loss of activity Mus musculus
2.7.1.25 D89E 75% loss of activity Mus musculus
2.7.1.25 D89R almost complete loss of activity Mus musculus
2.7.1.25 DN89ND switch mutant, almost complete loss of activity Mus musculus
2.7.1.25 G88A less than 30% loss of kinase activity Mus musculus
2.7.1.25 G88D almost complete loss of activity Mus musculus
2.7.1.25 G88R almost complete loss of activity Mus musculus
2.7.1.25 K97A no effect on activity Mus musculus
2.7.1.25 LD86DL switch mutant, almost complete loss of activity Mus musculus
2.7.1.25 N90A 30% loss of kinase activity Mus musculus
2.7.1.25 N90Q 30% loss of kinase activity Mus musculus
2.7.1.25 R92A complete loss of kinase activity Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.25 ATP + adenosine 5'-phosphosulfate Mus musculus synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate, enzyme defect causes brachymorphism, a severe growth disorder affecting skeletal elements as well as other physiological processes ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25 recombinant protein using His-tag Mus musculus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.25 0.000298
-
 wild type activity, specific activities given for all mutant enzymes Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5'-phosphosulfate
-
 Mus musculus ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?
2.7.1.25 ATP + adenosine 5'-phosphosulfate synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate, enzyme defect causes brachymorphism, a severe growth disorder affecting skeletal elements as well as other physiological processes Mus musculus ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.25 More bifunctional enzyme with ATP sulfurylase activity and adenosine phosphosulfate kinase activity, catalyzes the formation of 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate Mus musculus