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Literature summary extracted from

  • Rapacciuolo, A.; Suvarna, S.; Barki-Harrington, L.; Luttrell, L.M.; Cong, M.; Lefkowitz, R.J.; Rockman, H.A.
    Protein kinase A and G protein-coupled receptor kinase phosphorylation mediates beta-1 adrenergic receptor endocytosis through different pathways (2003), J. Biol. Chem., 278, 35403-35411.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.11 cAMP dependent on Homo sapiens
2.7.11.11 additional information beta-arrestin is not required for phosphorylation activity and receptor substrate endocytosis Homo sapiens
2.7.11.15 beta-arrestin required for phosphorylation activity and receptor substrate endocytosis Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.11 additional information construction and expression of a mouse mutant beta-adrenergic receptor lacking the PKA phosphorylation sites in HEK-293 cells, and coexpression of murine GFP-tagged beta-arrestin Homo sapiens
2.7.11.15 additional information construction and expression of a mouse mutant beta-adrenergic receptor lacking the GRK phosphorylation sites in HEK-293 cells, and coexpression of murine GFP-tagged beta-arrestin Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.11 H-89
-
 Homo sapiens
2.7.11.11 additional information caveolae inhibitors inhibit the internalization of the beta-adrenergic receptor by PKA Homo sapiens
2.7.11.15 additional information clathrin inhibitors inhibit the internalization of the beta-adrenergic receptor by GRK Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.11 cytosol
-
 Homo sapiens 5829
-
2.7.11.15 cytosol
-
 Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.11 ATP + beta-adrenergic receptor Homo sapiens PKA phosphorylation mediates beta-1 adrenergic receptor endocytosis via the caveolae pathway ADP + phosphorylated beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Homo sapiens G protein-coupled receptor kinase phosphorylation mediates beta-1 adrenergic receptor endocytosis via clathrin-coated pits ADP + phosphorylated beta-adrenergic receptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Homo sapiens
-
-
-
2.7.11.15 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.11 HEK-293 cell
-
 Homo sapiens
-
2.7.11.15 HEK-293 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.11 additional information
-
-
 Homo sapiens
2.7.11.15 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 ATP + beta-adrenergic receptor PKA phosphorylation mediates beta-1 adrenergic receptor endocytosis via the caveolae pathway Homo sapiens ADP + phosphorylated beta-adrenergic receptor
-
 ?
2.7.11.11 ATP + beta-adrenergic receptor mouse wild-type receptor, cytosolic phosphorylation domain near the transmembrane domains of the substrate, overview Homo sapiens ADP + phosphorylated beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor G protein-coupled receptor kinase phosphorylation mediates beta-1 adrenergic receptor endocytosis via clathrin-coated pits Homo sapiens ADP + phosphorylated beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor mouse wild-type receptor, cytosolic phosphorylation domain of the substrate, overview Homo sapiens ADP + phosphorylated beta-adrenergic receptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
-
 Homo sapiens
2.7.11.11 protein kinase A
-
 Homo sapiens
2.7.11.15 G protein-coupled receptor kinase
-
 Homo sapiens
2.7.11.15 GRK
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.11 37
-
 assay at Homo sapiens
2.7.11.15 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.11 7.4
-
 assay at Homo sapiens
2.7.11.15 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP
-
 Homo sapiens
2.7.11.15 ATP
-
 Homo sapiens