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Literature summary extracted from

  • Sun, Y.; Wilson, M.P.; Majerus, P.W.
    Inositol 1,3,4-trisphosphate 5/6-kinase associates with the COP9 signalosome by binding to CSN1 (2002), J. Biol. Chem., 277, 45759-45764.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.159 CSN1 the enzyme associates with the COP9 signalosome, an complex of 8 proteins, by binding to CSN1, activates Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.159 expression of the enzyme tagged with 6 repeats of Myc at the N-terminus in Spodoptera frugiperda Sf21 cells using the baculovirus infection system, expression of His6-tagged and of FLAG-tagged enzyme in Escherichia coli strain BL21(DE3), co-overexpression of the enzyme with CSN1 in HeLa cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.159 curcumin inhibits both the free enzyme and the COP9 signalosome complex-associated kinase Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.159 additional information
-
 additional information kinetics, recombinant enzyme with CSN1 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.159 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.159 60000
-
 COP9 signalosome, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.159 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.159 recombinant enzyme from insect Sf21 cells to homogeneity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate substrate of the free enzyme and the COP9 signalosome complex Homo sapiens ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol-1,3,4-trisphosphate substrate of the free enzyme and the COP9 signalosome complex Homo sapiens ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
 ?
2.7.1.159 ATP + c-Jun substrate of the free enzyme and the COP9 signalosome complex, which both show no activity with the 1-79 residue fragment of c-Jun Homo sapiens ADP + phosphorylated c-Jun
-
 ?
2.7.1.159 ATP + IkappaBalpha substrate of the free enzyme and the COP9 signalosome complex Homo sapiens ADP + phosphorylated IkappaBalpha
-
 ?
2.7.1.159 ATP + p53 substrate of the free enzyme and the COP9 signalosome complex Homo sapiens ADP + phosphorylated p53
-
 ?
2.7.1.159 additional information the enzyme associates with the COP9 signalosome, an complex of 8 proteins, by binding to CSN1 Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.159 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.159 5
-
 1D-myo-inositol-1,3,4-trisphosphate pH 7.6, 37°C, in absence of CSN1, first order rate constant Homo sapiens
2.7.1.159 20
-
 1D-myo-inositol-1,3,4-trisphosphate pH 7.6, 37°C, in presence of CSN1, first order rate constant Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.159 7.6
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.159 ATP
-
 Homo sapiens