EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.93 | F229L | turnover number for adipyl-7-aminodesacetoxycephalosporanic acid is nealy identical to wild-type value, 1.8fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 1.4fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 1.45fold increase in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme | Pseudomonas sp. |
3.5.1.93 | F375L | 1.5fold increase in turnover number for adipyl-7-aminodesacetoxycephalosporanic acid, 1.2fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 3.9fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 8.2fold increase in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme. Improved activity ratio for adipyl-7-aminodesacetoxycephalosporanic acid to glutaryl-7-aminocephalosporanic acid of the mutant enzyme is a consequence of a decreased catalytic efficiency towards glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. |
3.5.1.93 | M271V/Q291K/T374S | 1.3fold increase in turnover number for adipyl-7-aminodesacetoxycephalosporanic acid, 1.4fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 1.2fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 1.3fold increase in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme | Pseudomonas sp. |
3.5.1.93 | N266H | 1.2fold increase in turnover number for adipyl-7-aminodesacetoxycephalosporanic acid, 6.9fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 1.3fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 2fold decrease in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme. Nearly 10fold improved catalytic efficiency on adipyl-7-aminodesacetoxycephalosporanic acid, resulting from a 50% increase in turnover-number and a 6fold decrease in KM-value without decreasing the catalytic efficiency on glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. |
3.5.1.93 | N266S | 1.4fold decrease in turnover number for adipyl-7-aminodesacetoxycephalosporanic acid, 2.3fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 1.95fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 2.8fold increase in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.93 | 0.016 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme N266H | Pseudomonas sp. | |
3.5.1.93 | 0.033 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, wild-type enzyme | Pseudomonas sp. | |
3.5.1.93 | 0.043 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme M271V/Q291K/T374S | Pseudomonas sp. | |
3.5.1.93 | 0.048 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F229L | Pseudomonas sp. | |
3.5.1.93 | 0.094 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme N266S | Pseudomonas sp. | |
3.5.1.93 | 0.14 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | pH 7.5, 37°C, mutant enzyme N266H | Pseudomonas sp. | |
3.5.1.93 | 0.27 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375L | Pseudomonas sp. | |
3.5.1.93 | 0.42 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | pH 7.5, 37°C, mutant enzyme N266S | Pseudomonas sp. | |
3.5.1.93 | 0.52 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | pH 7.5, 37°C, mutant enzyme F229L | Pseudomonas sp. | |
3.5.1.93 | 0.7 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | pH 7.5, 37°C, mutant enzyme M271V/Q291K/T374S | Pseudomonas sp. | |
3.5.1.93 | 0.82 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375L | Pseudomonas sp. | |
3.5.1.93 | 0.96 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | pH 7.5, 37°C, wild-type enzyme | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.93 | Pseudomonas sp. | P07662 | SY-77 | - |
3.5.1.93 | Pseudomonas sp. SY-77 | P07662 | SY-77 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.93 | adipyl-7-aminodesacetoxycephalosporanic acid + H2O | - |
Pseudomonas sp. | deacetoxycephalosporanic acid + adipate | - |
? | |
3.5.1.93 | adipyl-7-aminodesacetoxycephalosporanic acid + H2O | - |
Pseudomonas sp. SY-77 | deacetoxycephalosporanic acid + adipate | - |
? | |
3.5.1.93 | glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. | 7-aminocephalosporanate + glutarate | - |
? | |
3.5.1.93 | glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. SY-77 | 7-aminocephalosporanate + glutarate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.93 | 0.34 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | pH 7.5, 37°C, mutant enzyme N266S | Pseudomonas sp. | |
3.5.1.93 | 0.46 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | pH 7.5, 37°C, wild-type enzyme | Pseudomonas sp. | |
3.5.1.93 | 0.47 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | pH 7.5, 37°C, mutant enzyme F229L | Pseudomonas sp. | |
3.5.1.93 | 0.55 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | pH 7.5, 37°C, mutant enzyme N266H | Pseudomonas sp. | |
3.5.1.93 | 0.61 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | pH 7.5, 37°C, mutant enzyme M271V/Q291K/T374S | Pseudomonas sp. | |
3.5.1.93 | 0.67 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375L | Pseudomonas sp. | |
3.5.1.93 | 1.1 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F375L | Pseudomonas sp. | |
3.5.1.93 | 2.2 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme N266S | Pseudomonas sp. | |
3.5.1.93 | 3.1 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme F229L | Pseudomonas sp. | |
3.5.1.93 | 3.3 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme N266H | Pseudomonas sp. | |
3.5.1.93 | 3.5 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, mutant enzyme M271V/Q291K/T374S | Pseudomonas sp. | |
3.5.1.93 | 4.3 | - |
glutaryl-7-aminocephalosporanic acid | pH 7.5, 37°C, wild-type enzyme | Pseudomonas sp. |