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Literature summary extracted from

  • Patel, H.M.; Kraszewski, J.L.; Mukhopadhyay, B.
    The phosphoenolpyruvate carboxylase from Methanothermobacter thermautotrophicus has a novel structure (2004), J. Bacteriol., 186, 5129-5137.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 ADP 10mM, 57% inhibition in presence of 5 mM Mg2+, 46% inhibition in presence of 15 mM Mg2+ Methanothermobacter thermautotrophicus
4.1.1.31 AMP 10mM, 16% inhibition in presence of 5 mM Mg2+, 12% inhibition in presence of 15 mM Mg2+ Methanothermobacter thermautotrophicus
4.1.1.31 ATP 10mM, 92% inhibition in presence of 5 mM Mg2+, 65% inhibition in presence of 15 mM Mg2+ Methanothermobacter thermautotrophicus
4.1.1.31 GTP 10mM, 58% inhibition in presence of 5 mM Mg2+, 15% inhibition in presence of 15 mM Mg2+ Methanothermobacter thermautotrophicus
4.1.1.31 KCl 0.05-1.0 M, 60% inhibition at 0.25 M, 27% inhibition at 0.1 M Methanothermobacter thermautotrophicus
4.1.1.31 L-aspartate 10 mM, 41% inhibition in presence of 5 mM Mg2+ or 15 mM Mg2+ Methanothermobacter thermautotrophicus
4.1.1.31 NaCl 0.05-1.0 M, 60% inhibition at 0.25 M, 27% inhibition at 0.1 M Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 0.46
-
 phosphoenolpyruvate
-
 Methanothermobacter thermautotrophicus
4.1.1.31 1.8
-
 HCO3-
-
 Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 Mg2+ required Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 50000
-
 4 * 50000 Methanothermobacter thermautotrophicus
4.1.1.31 277000
-
 gel filtration Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
 Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 additional information
-
-
 Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + HCO3-
-
 Methanothermobacter thermautotrophicus phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 homotetramer 4 * 50000 Methanothermobacter thermautotrophicus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.31 PpcA
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.31 62
-
-
 Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 6.8
-
-
 Methanothermobacter thermautotrophicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.31 6.3 7.2 pH 6.3: 5% of maximal activity, pH 7.2: 92% of maximal activity Methanothermobacter thermautotrophicus