EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.35 | expressed as His-tag fusion protein in Escherichia coli BL21(DE3) | Bacillus subtilis |
2.7.1.49 | gene annotated ThiD, instead of YjbV, subcloning in Escherichia coli strain DH5alpha, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.35 | 0.047 | - |
pyridoxal | pH 8, 37°C | Bacillus subtilis | |
2.7.1.35 | 2.03 | - |
4-Amino-5-hydroxymethyl-2-methylpyrimidine | pH 8, 37°C | Bacillus subtilis | |
2.7.1.49 | additional information | - |
additional information | - |
Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.49 | Mg2+ | MgCl2 at 2 mM | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.35 | ATP + pyridoxal | Bacillus subtilis | generates the active form of vitamin B6, which serves as cofactor for many enzymes | ADP + pyridoxal 5'-phosphate | - |
? | |
2.7.1.49 | ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine | Bacillus subtilis | - |
ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine | - |
? | |
2.7.1.49 | ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine | Bacillus subtilis | enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase | ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine | - |
? | |
2.7.1.49 | additional information | Bacillus subtilis | the enzyme has both a biosynthetic and a salvage pathway function | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.35 | Bacillus subtilis | - |
- |
- |
2.7.1.49 | Bacillus subtilis | - |
gene annotated ThiD, instead of YjbV | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.35 | recombinant protein using His-tag | Bacillus subtilis |
2.7.1.49 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.35 | ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine | - |
Bacillus subtilis | ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine | - |
? | |
2.7.1.35 | ATP + pyridoxal | - |
Bacillus subtilis | ADP + pyridoxal 5'-phosphate | - |
? | |
2.7.1.35 | ATP + pyridoxal | generates the active form of vitamin B6, which serves as cofactor for many enzymes | Bacillus subtilis | ADP + pyridoxal 5'-phosphate | - |
? | |
2.7.1.35 | ATP + pyridoxamine | - |
Bacillus subtilis | ADP + pyridoxamine 5'-phosphate | - |
? | |
2.7.1.35 | ATP + pyridoxine | - |
Bacillus subtilis | ADP + pyridoxine 5'-phosphate | - |
? | |
2.7.1.49 | ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine | - |
Bacillus subtilis | ADP + 4-amino-5-phosphomethyl-2-methylpyrimidine | - |
? | |
2.7.1.49 | ATP + 4-amino-5-phosphomethyl-2-methylpyrimidine | enzyme also performs the reaction of EC 2.7.4.7, phosphomethylpyrimidine kinase | Bacillus subtilis | ADP + 4-amino-5-diphosphomethyl-2-methylpyrimidine | - |
? | |
2.7.1.49 | additional information | the enzyme has both a biosynthetic and a salvage pathway function | Bacillus subtilis | ? | - |
? | |
2.7.1.49 | additional information | no activity with pyridoxal, pyridoxine, or pyridoxamine | Bacillus subtilis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.35 | PdxK | formerly ThiD | Bacillus subtilis |
2.7.1.49 | 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase | - |
Bacillus subtilis |
2.7.1.49 | 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase | - |
Bacillus subtilis |
2.7.1.49 | ThiD | - |
Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.49 | 37 | - |
assay at | Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.35 | 0.032 | - |
pyridoxal | pH 8, 37°C | Bacillus subtilis | |
2.7.1.35 | 0.36 | - |
4-Amino-5-hydroxymethyl-2-methylpyrimidine | pH 8, 37°C | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.49 | 8 | - |
assay at | Bacillus subtilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.49 | ATP | - |
Bacillus subtilis |