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Literature summary extracted from

  • Hove-Jensen, B.; Rosenkrantz, T.J.; Haldimann, A.; Wanner, B.L.
    Escherichia coli phnN, encoding ribose 1,5-bisphosphokinase activity (phosphoribosyl diphosphate forming): dual role in phosphonate degradation and NAD biosynthesis pathways (2003), J. Bacteriol., 185, 2793-2801.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.23 into pSK50-delta A2 and then subcloned into pCAH56 and into pAH126 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.23 21410
-
 calculated from the deduced nucleotide sequence of the allele specifying the histidine-tailed polypeptide Escherichia coli
2.7.4.23 23500
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.23 Escherichia coli P16690 K-12 strains
-
2.7.4.23 Escherichia coli P16690 phnN
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.23 purification of histidine-tailed ribose 1,5-bisphosphokinase, centrifugation, ultrasonic treatment, application to Co2+-containing Talon metal affinity resin, purified almost to homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.23 0.01
-
 acitivity very low in a crude extract of strain HO1088/pTR553 before addition of IPTG Escherichia coli
2.7.4.23 0.03
-
 acitivity in a crude extract of strain HO1088/pHO500 after addition of IPTG Escherichia coli
2.7.4.23 0.12
-
 acitivity in a crude extract of strain HO1088/pTR553 after addition of IPTG Escherichia coli
2.7.4.23 8.3
-
 purified PhnN protein Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.23 ATP + ribose 1,5-bisphosphate
-
 Escherichia coli 5-phospho-alpha-D-ribose 1-diphosphate + ADP
-
 ?
2.7.4.23 additional information no catalysis with ATP + ribose, ATP + ribose 1-phosphate or ATP + ribose 5-phosphate detected Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.23 PhnN protein
-
 Escherichia coli
2.7.4.23 ribose 1,5-bisphosphokinase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.23 ATP
-
 Escherichia coli
2.7.4.23 additional information GTP cannot act as a phosphoryl donor Escherichia coli