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Literature summary extracted from

  • Wall, L.; Meighen, E.A.
    Subunit structure of the fatty acid reductase complex from Photobacterium phosphoreum (1986), Biochemistry, 25, 4315-4321.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.19 Mg2+ required for formation of acyl-AMP Photobacterium phosphoreum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.50 205000 223000 reductase component of fatty acid reductase enzyme complex, gel filtration, SDS-PAGE after cross-linkage with bis(sulfosuccinimidyl)suberate Photobacterium phosphoreum
1.2.1.50 450000
-
 fatty acid reductase enzyme complex composed of synthetase (s), reductase (r) and transferase (t), molar ratio s: r: t is 1: 1: 0.5, gel filtration Photobacterium phosphoreum
6.2.1.19 additional information
-
 450000, MW of the fatty acid reductase complex, gel filtration Photobacterium phosphoreum
6.2.1.19 45000
-
 1 * 45000, SDS-PAGE Photobacterium phosphoreum
6.2.1.19 66000
-
 gel filtration Photobacterium phosphoreum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.50 tetradecanoyl-CoA + NADPH + H+ Photobacterium phosphoreum
-
 tetradecanal + CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.50 Photobacterium phosphoreum
-
-
-
6.2.1.19 Photobacterium phosphoreum
-
 together with acyl-CoA reductase, EC 1.2.1.50, and acyl-transferase, the acyl-protein synthetase forms the fatty acid reductase complex. The molar ratio of these enzymes in the complex is: 1.0:1.0:0.5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.50 partial Photobacterium phosphoreum
6.2.1.19
-
 Photobacterium phosphoreum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.50 tetradecanoyl-CoA + NADPH + H+
-
 Photobacterium phosphoreum tetradecanal + CoA + NADP+
-
 ?
6.2.1.19 ATP + myristic acid + protein
-
 Photobacterium phosphoreum AMP + diphosphate + myristoyl-protein thiolester
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.50 tetramer 4 * 50000-58000, reductase component of fatty acid reductase enzyme complex, SDS-PAGE Photobacterium phosphoreum
6.2.1.19 monomer 1 * 45000, SDS-PAGE Photobacterium phosphoreum