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Literature summary extracted from

  • He, Z.; Stigers Lavoie, K.D.; Bartlett, P.A.; Toney, M.D.
    Conservation of mechanism in three chorismate-utilizing enzymes (2004), J. Am. Chem. Soc., 126, 2378-2385.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.85 expression with His-tag Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.85 K274A 265fold reduction of kcat-value Escherichia coli
2.6.1.85 K274R 640fold reduction of kcat-value Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.85 (5R,6R)-4-ammonio-5-((1-carboxylatovinyl)oxy)-6-hydroxycyclohex-1-ene-1-carboxylate transition-state analogue, competitive Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.85 0.013
-
 chorismate wild-type, cosubstrate L-glutamine, pH 8.5, 25°C Escherichia coli
2.6.1.85 0.058
-
 chorismate wild-type, cosubstrate NH4+, pH 8.5, 25°C Escherichia coli
2.6.1.85 0.097
-
 chorismate mutant K274A, cosubstrate NH4+, pH 8.5, 25°C Escherichia coli
2.6.1.85 145
-
 NH4+ wild-type, pH 8.5, 25°C Escherichia coli
2.6.1.85 169
-
 NH4+ mutant K274A, pH 8.5, 25°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.85 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.85 chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate mechanism includes nucleophile addition to C2 of chorismate in an SN2'' process Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.85 chorismate + L-glutamine
-
 Escherichia coli 4-amino-4-deoxychorismate + L-glutamate
-
 ?
2.6.1.85 chorismate + NH4+
-
 Escherichia coli 4-amino-4-deoxychorismate + H2O + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.85 0.000098
-
 chorismate mutant K274R, cosubstrate NH4+, pH 8.5, 25°C Escherichia coli
2.6.1.85 0.00083
-
 chorismate mutant K274R, cosubstrate L-glutamine, pH 8.5, 25°C Escherichia coli
2.6.1.85 0.002
-
 chorismate mutant K274A, cosubstrate L-glutamine, pH 8.5, 25°C Escherichia coli
2.6.1.85 0.026
-
 chorismate mutant K274A, cosubstrate NH4+, pH 8.5, 25°C Escherichia coli
2.6.1.85 0.032
-
 chorismate wild-type, cosubstrate NH4+, pH 8.5, 25°C Escherichia coli
2.6.1.85 0.53
-
 chorismate wild-type, cosubstrate L-glutamine, pH 8.5, 25°C Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.85 0.0007
-
 (5R,6R)-4-ammonio-5-((1-carboxylatovinyl)oxy)-6-hydroxycyclohex-1-ene-1-carboxylate mutant K274A, pH 8.5, 25°C Escherichia coli
2.6.1.85 0.0056
-
 (5R,6R)-4-ammonio-5-((1-carboxylatovinyl)oxy)-6-hydroxycyclohex-1-ene-1-carboxylate wild-type, pH 8.5, 25°C Escherichia coli