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Literature summary extracted from

  • Kohjitani, A.; Fuda, H.; Hanyu, O.; Strott, C.A.
    Cloning, characterization and tissue expression of rat SULT2B1a and SULT2B1b steroid/sterol sulfotransferase isoforms: Divergence of the rat SULT2B1 gene structure from orthologous human and mouse genes (2005), Gene, 367, 66-73.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.2
-
 Rattus norvegicus
2.8.2.2 gene SULT2B1, isozymes SULT2B1a and SULT2B1b as a result of an alternative exon I, DNA and amino acid sequence determination and analysis, genetic structure, expression analysis, comparison of sequence and genetic structure, overview, overexpression of GST-tagged isozymes in Escherichia coli strain BL21(DE3) Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.2 additional information
-
 additional information steady-state kinetic analysis of recombinant isozymes SULT2B1a and SULT2B1b Rattus norvegicus
2.8.2.2 0.0017
-
 cholesterol pH 7.5, 37°C, recombinant isozyme SULT2B1b Rattus norvegicus
2.8.2.2 0.0089
-
 pregnenolone pH 7.5, 37°C, recombinant isozyme SULT2B1a Rattus norvegicus
2.8.2.2 0.0179
-
 dehydroepiandrosterone pH 7.5, 37°C, recombinant isozyme SULT2B1a Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.2 Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.2 38319
-
 x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation Rattus norvegicus
2.8.2.2 42178
-
 x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.2 3'-phosphoadenylyl sulfate + cholesterol Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylyl sulfate + dehydroepiandrosterone Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylyl sulfate + pregnenolone Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.2 Rattus norvegicus Q29YR5 isoform SULT2B1a
-
2.8.2.2 Rattus norvegicus Q29YR5 isoform SULT2B1b
-
2.8.2.2 Rattus norvegicus Q29YR5 SULT2B1; Sprague-Dawley rats, gene SULT2B1, isozymes SULT2B1a and SULT2B1b as a result of an alternative exon I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.2
-
 Rattus norvegicus
2.8.2.2 recombinant GST-tagged isozymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.2 brain isoform SULT2B1a Rattus norvegicus
-
2.8.2.2 brain SULT2B1a is only expressed in brain and testis Rattus norvegicus
-
2.8.2.2 intestine isoform SULT2B1b Rattus norvegicus
-
2.8.2.2 intestine main expression of SULT2B1b Rattus norvegicus
-
2.8.2.2 kidney isoform SULT2B1b Rattus norvegicus
-
2.8.2.2 kidney main expression of SULT2B1b Rattus norvegicus
-
2.8.2.2 additional information tissue distribution of isozymes SULT2B1a and SULT2B1b expression, overview Rattus norvegicus
-
2.8.2.2 skin isoform SULT2B1b Rattus norvegicus
-
2.8.2.2 skin main expression of SULT2B1b Rattus norvegicus
-
2.8.2.2 testis isoform SULT2B1a Rattus norvegicus
-
2.8.2.2 testis SULT2B1a is only expressed in brain and testis Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.2 3'-phosphoadenylyl sulfate + cholesterol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylyl sulfate + dehydroepiandrosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylyl sulfate + pregnenolone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + cholesterol activity is higher than with pregnenolone, isoform SULT2B1b Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + cholesterol poor activity, isoform SULT2B1a Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + pregnenolone isoform SULT2B1a Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.2 3'-phosphoadenylylsulfate + pregnenolone isoform SULT2B1b Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.2 additional information no activity with dehydroepiandrosterone, isoform SULT2b1a Rattus norvegicus ?
-
 ?
2.8.2.2 additional information no activity with dehydroepiandrosterone, isoform SULT2b1b Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.2 ? x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.8.2.2 steroid/sterol sulfotransferase
-
 Rattus norvegicus
2.8.2.2 SULT2B1
-
 Rattus norvegicus
2.8.2.2 SULT2B1a
-
 Rattus norvegicus
2.8.2.2 SULT2B1b
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.2 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.2 7.5
-
 assay at Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.2 Rattus norvegicus sequence calculation, SULT2B1b
-
 5.12
2.8.2.2 Rattus norvegicus sequence calculation, SULT2B1a
-
 5.93