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Literature summary extracted from
- PknH, a transmembrane Hank's type serine/threonine kinase from Mycobacterium tuberculosis is differentially expressed under stress conditions (2004), FEMS Microbiol. Lett., 233, 107-113.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.7.11.1 | gene Rv1266c or pknH, overexpression of wild-type and mutant GST-fusion PknH in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 2.7.11.1 | K45M | site-directed mutagenesis, inactive mutant | Mycobacterium tuberculosis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.11.1 | H-7 | - |
Mycobacterium tuberculosis |  |
| 2.7.11.1 | additional information | enzyme expression level is decreased under low pH and heat shock conditions | Mycobacterium tuberculosis | |
| 2.7.11.1 | staurosporine | - |
Mycobacterium tuberculosis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.7.11.1 | membrane | PknH is a transmembrane protein | Mycobacterium tuberculosis | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.11.1 | Mg2+ | Mg2+ or Mn2+, dependent on | Mycobacterium tuberculosis | |
| 2.7.11.1 | Mn2+ | Mg2+ or Mn2+, dependent on | Mycobacterium tuberculosis | |
| 2.7.11.1 | additional information | no activity with cations other than Mg2+ or Mn2+ | Mycobacterium tuberculosis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.11.1 | 68000 | - |
x * 97000, recombinant GST-fusion PknH, SDS-PAGE, x * 68000, non-tagged PknH, SDS-PAGE | Mycobacterium tuberculosis |
| 2.7.11.1 | 97000 | - |
x * 97000, recombinant GST-fusion PknH, SDS-PAGE, x * 68000, non-tagged PknH, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.11.1 | additional information | Mycobacterium tuberculosis | enzyme is involved in stress response and adaptation to environmental changes in mycobacteria | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.11.1 | Mycobacterium tuberculosis | - |
strains H37Rv and H37Ra, PknH is specific for mycobacteria of the Mycobacterium tuberculosis complex | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 2.7.11.1 | phosphoprotein | PknH performs autophosphorylation, mutant K45M is inactive | Mycobacterium tuberculosis |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.11.1 | recombinant wild-type and mutant GST-fusion PknH from Escherichia coli by glutathione affinity chromatography | Mycobacterium tuberculosis |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 2.7.11.1 | ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | Lys45 is important for PknH catalytic and autophosphorylation activity | Mycobacterium tuberculosis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.7.11.1 | additional information | enzyme expression level under stress conditions, overview | Mycobacterium tuberculosis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.11.1 | ATP + histone | - |
Mycobacterium tuberculosis | ADP + phosphorylated histone | - |
? | |
| 2.7.11.1 | ATP + myelin basic protein | - |
Mycobacterium tuberculosis | ADP + phosphorylated myelin basic protein | - |
? | |
| 2.7.11.1 | additional information | enzyme is involved in stress response and adaptation to environmental changes in mycobacteria | Mycobacterium tuberculosis | ? | - |
? | |
| 2.7.11.1 | additional information | PknH performs autophosphorylation | Mycobacterium tuberculosis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.11.1 | ? | x * 97000, recombinant GST-fusion PknH, SDS-PAGE, x * 68000, non-tagged PknH, SDS-PAGE | Mycobacterium tuberculosis |
| 2.7.11.1 | More | PknH contains 11 subdomains, schematic structure, overview | Mycobacterium tuberculosis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.7.11.1 | Hank's type serine/threonine kinase | - |
Mycobacterium tuberculosis |
| 2.7.11.1 | PknH | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.11.1 | 37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.7.11.1 | 7.4 | - |
assay at | Mycobacterium tuberculosis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.11.1 | ATP | - |
Mycobacterium tuberculosis | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 2.7.11.1 | Mycobacterium tuberculosis | amino acid sequence calculation | - |
6.3 |
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