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Literature summary extracted from

  • Caniuguir, A.; Cabrera, R.; Baez, M.; Vasquez, C.C.; Babul, J.; Guixe, V.
    Role of Cys-295 on subunit interactions and allosteric regulation of phosphofructokinase-2 from Escherichia coli (2005), FEBS Lett., 579, 2313-2318.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.105 expression of wild-type and mutant enzymes in enzyme-deficient strain DF1020 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.105 C238A site-directed mutagenesis, the mutation does not affect the kinetic parameters, allosteric inhibition, dimer stability, nor oligomeric structure of the enzyme Escherichia coli
2.7.1.105 C238F site-directed mutagenesis, the mutation does not affect the kinetic parameters, allosteric inhibition, dimer stability, nor oligomeric structure of the enzyme Escherichia coli
2.7.1.105 C295A site-directed mutagenesis, decreased kcat and increased Km for ATP and beta-D-fructose 6-phosphate compared to the wild-type enzyme Escherichia coli
2.7.1.105 C295F site-directed mutagenesis, decreased kcat and increased Km for ATP and beta-D-fructose 6-phosphate compared to the wild-type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.105 MgATP2- allosteric inhibition, important regulation of in vivo carbohydrate metabolism under gluconeogenic conditions Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.105 additional information
-
 additional information kinetics of recombinant wild-type and mutant enzymes Escherichia coli
2.7.1.105 0.0107
-
 MgATP2- recombinant mutant C295F Escherichia coli
2.7.1.105 0.017
-
 MgATP2- recombinant wild-type enzyme and mutant C238A Escherichia coli
2.7.1.105 0.021
-
 MgATP2- recombinant mutant C238F Escherichia coli
2.7.1.105 0.031
-
 beta-D-fructose 6-phosphate recombinant wild-type enzyme and mutant C238A Escherichia coli
2.7.1.105 0.047
-
 beta-D-fructose 6-phosphate recombinant mutant C238F Escherichia coli
2.7.1.105 0.061
-
 MgATP2- recombinant mutant C295A Escherichia coli
2.7.1.105 0.081
-
 beta-D-fructose 6-phosphate recombinant mutant C295F Escherichia coli
2.7.1.105 0.088
-
 beta-D-fructose 6-phosphate recombinant mutant C295A Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 Mg2+ as MgATP2- Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate Escherichia coli
-
 ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 additional information Escherichia coli Cys-295 is involved in allosteric regulation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.105 recombinant wild-type and mutant enzymes from enzyme-deficient strain DF1020 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Escherichia coli ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 MgATP2- + beta-D-fructose 6-phosphate
-
 Escherichia coli ?
-
 ?
2.7.1.105 additional information Cys-295 is involved in allosteric regulation Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.105 dimer active form Escherichia coli
2.7.1.105 More oligomeric state of the enzyme plays a role in enzyme activity and regulation, alterations occur via binding of MgATP2- which mediates dimer-dimer interactions, the dimeric state of the enzyme is critical for stability and activity of the enzyme, Cys-295 plays a role in subunit interactions, overview Escherichia coli
2.7.1.105 tetramer inactive form Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.1.105 Pfk-2
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.105 13
-
 beta-D-fructose 6-phosphate recombinant mutant C295A Escherichia coli
2.7.1.105 13
-
 MgATP2- recombinant mutant C295F Escherichia coli
2.7.1.105 39
-
 MgATP2- recombinant mutant C295A Escherichia coli
2.7.1.105 39
-
 beta-D-fructose 6-phosphate recombinant mutant C295F Escherichia coli
2.7.1.105 70
-
 MgATP2- recombinant mutants C238A and C238F Escherichia coli
2.7.1.105 70
-
 beta-D-fructose 6-phosphate recombinant mutants C238A and C238F Escherichia coli
2.7.1.105 90
-
 MgATP2- recombinant wild-type enzyme Escherichia coli
2.7.1.105 90
-
 beta-D-fructose 6-phosphate recombinant wild-type enzyme Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.105 ATP as MgATP2- Escherichia coli