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Literature summary extracted from

  • Strisovsky, K.; Jiraskova, J.; Barinka, C.; Majer, P.; Rojas, C.; Slusher, B.S.; Konvalinka, J.
    Mouse brain serine racemase catalyzes specific elimination of L-serine to pyruvate (2003), FEBS Lett., 535, 44-48.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.17 Co2+ inhibition of both activities Mus musculus
4.3.1.17 Cu2+ inhibition of both activities Mus musculus
4.3.1.17 EDTA inhibition of both activities Mus musculus
4.3.1.17 Fe2+ slight inhibition of both activities Mus musculus
4.3.1.17 Ni2+ slight inhibition of both activities Mus musculus
4.3.1.17 Zn2+ inhibition of both activities Mus musculus
5.1.1.10 Co2+ inhibition of both activities Mus musculus
5.1.1.10 Cu2+ inhibition of both activities Mus musculus
5.1.1.10 EDTA inhibition of both activities Mus musculus
5.1.1.10 Fe2+ slight inhibition of both activities Mus musculus
5.1.1.10 Ni2+ slight inhibition of both activities Mus musculus
5.1.1.10 Zn2+ inhibition of both activities Mus musculus
5.1.1.18 Co2+
-
 Mus musculus
5.1.1.18 Cu2+
-
 Mus musculus
5.1.1.18 EDTA
-
 Mus musculus
5.1.1.18 Fe2+ slight inhibition of both activities Mus musculus
5.1.1.18 Ni2+ slight inhibition of both activities Mus musculus
5.1.1.18 Zn2+
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.17 30
-
 L-serine pH 8.0, 37°C, racemization reaction Mus musculus
4.3.1.17 49
-
 D-serine pH 8.0, 37°C, racemization reaction Mus musculus
4.3.1.17 75
-
 L-serine pH 8.0, 37°C, elimination reaction Mus musculus
4.3.1.17 75
-
 D-serine pH 8.0, 37°C, elimination reaction Mus musculus
5.1.1.10 30
-
 L-serine pH 8.0, 37°C, racemization reaction Mus musculus
5.1.1.10 49
-
 D-serine pH 8.0, 37°C, racemization reaction Mus musculus
5.1.1.18 30
-
 L-serine pH 8.0, 37°C, racemization reaction Mus musculus
5.1.1.18 49
-
 D-serine pH 8.0, 37°C, racemization reaction Mus musculus
5.1.1.18 75
-
 L-serine pH 8.0, 37°C, elimination reaction Mus musculus
5.1.1.18 75
-
 D-serine pH 8.0, 37°C, elimination reaction Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.17 Ca2+
-
 Mus musculus
4.3.1.17 Mg2+ required, both activities Mus musculus
4.3.1.17 Mn2+
-
 Mus musculus
5.1.1.10 Ca2+
-
 Mus musculus
5.1.1.10 Mg2+ required, both activities Mus musculus
5.1.1.10 Mn2+
-
 Mus musculus
5.1.1.18 Ca2+
-
 Mus musculus
5.1.1.18 Mg2+ required, both activities Mus musculus
5.1.1.18 Mn2+
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.17 37000
-
 2 * 37000, SDS-PAGE Mus musculus
4.3.1.17 78000
-
 gel filtration Mus musculus
5.1.1.10 37000
-
 2 * 37000, SDS-PAGE Mus musculus
5.1.1.10 78000
-
 gel filtration Mus musculus
5.1.1.18 37000
-
 2 * 37000, SDS-PAGE Mus musculus
5.1.1.18 78000
-
 gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.17 Mus musculus Q9QZX7 bifunctional enzyme, racemization of serine and elimination of L-serine and L-serine-O-sulfate to form pyruvate
-
5.1.1.10 Mus musculus
-
 bifunctional enzyme, racemization of serine and elimination of L-serine and L-serine-O-sulfate to form pyruvate
-
5.1.1.18 Mus musculus Q9QZX7 bifunctional enzyme, racemization of serine and elimination of L-serine and L-serine-O-sulfate to form pyruvate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.17 recombinant enzyme expressed in insect cells Mus musculus
5.1.1.10 recombinant enzyme expressed in insect cells Mus musculus
5.1.1.18 recombinant enzyme expressed in insect cells Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.17 D-serine
-
 Mus musculus pyruvate + NH3
-
 ?
4.3.1.17 D-serine racemization reaction Mus musculus L-serine
-
 r
4.3.1.17 L-serine
-
 Mus musculus pyruvate + NH3
-
 ?
4.3.1.17 L-serine racemization reaction Mus musculus D-serine
-
 r
5.1.1.10 D-serine
-
 Mus musculus L-serine
-
 r
5.1.1.10 L-serine
-
 Mus musculus D-serine
-
 r
5.1.1.18 D-serine
-
 Mus musculus pyruvate + NH3
-
 ?
5.1.1.18 D-serine
-
 Mus musculus L-serine
-
 r
5.1.1.18 L-serine
-
 Mus musculus pyruvate + NH3
-
 ?
5.1.1.18 L-serine
-
 Mus musculus D-serine
-
 r

Subunits

EC Number Subunits Comment Organism
4.3.1.17 dimer 2 * 37000, SDS-PAGE Mus musculus
5.1.1.10 dimer 2 * 37000, SDS-PAGE Mus musculus
5.1.1.18 dimer 2 * 37000, SDS-PAGE Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.17 8 9.5
-
 Mus musculus
5.1.1.10 8 9.5
-
 Mus musculus
5.1.1.18 8 9.5
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.17 6.5
-
 negligible activity below Mus musculus
5.1.1.10 6.5
-
 negligible activity below Mus musculus
5.1.1.18 6.5
-
 negligible activity below Mus musculus