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Literature summary extracted from

  • Bucko-Justyna, M.; Lipinski, L.; Burgering, B.M.; Trzeciak, L.
    Characterization of testis-specific serine-threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling (2005), FEBS J., 272, 6310-6323.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.1 additional information insulin induces the phosphorylation of TSSK3, TSSK3 activation by phosphoinositide-dependent kinase-1 PDK1-dependent signalling, phosphorylation at Thr168 activates Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 expression of wild-type and mutant GST-fusion TSSK3s in Escherichia coli strain BL21, expression of Myc-tagged catalytic subunit of phosphoinositide-dependent kinase-1 in human 293T cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.1 K39R site-directed mutagenesis, inactive mutant Mus musculus
2.7.11.1 S166A site-directed mutagenesis, mutation of a T-loop residue, highly reduced kinase activity compared to the wild-type TSSK3, no autophosphorylation Mus musculus
2.7.11.1 S166D site-directed mutagenesis, mutation of a T-loop residue, reduced kinase activity compared to the wild-type TSSK3, no autophosphorylation Mus musculus
2.7.11.1 S166G site-directed mutagenesis, mutation of a T-loop residue, highly reduced kinase activity compared to the wild-type TSSK3, no autophosphorylation Mus musculus
2.7.11.1 T168A site-directed mutagenesis, mutation of a T-loop residue, inactive TSSK3 mutant, no autophosphorylation Mus musculus
2.7.11.1 T168D site-directed mutagenesis, mutation of a T-loop residue, unaltered kinase activity and reduced autophosphorylation compared to the wild-type TSSK3 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.1 0.145
-
 myelin basic protein pH 7.4, 30°C, recombinant TSSK3 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mg2+
-
 Mus musculus
2.7.11.1 Mn2+ preferred cation, optimal at 10 mM Mus musculus
2.7.11.1 additional information divalent cations are required for activity Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.1 ATP + testis-specific serine-threonine kinase 3 Mus musculus substrate of phosphoinositide-dependent kinase-1 leading to activation ADP + phosphorylated testis-specific serine-threonine kinase 3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.1 phosphoprotein TSSK3 performs autophosphorylation, TSSK3 is phosphorylated at Thr168 of the T-loop by recombinant Myc-tagged catalytic subunit of phosphoinositide-dependent kinase-1 leading to activation of TSSK3, TSSK3 can also be phosphorylated by cAMP-dependent protein kinase PKA, EC 2.7.11.11, but not by protein kinase B Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 recombinant wild-type and mutant GST-fusion TSSK3s by glutathione affinity chromatography, recombinant Myc-tagged catalytic subunit of phosphoinositide-dependent kinase-1 from human 293T cells Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.1 testis testis-specific serine-threonine kinase 3, i.e. TSSK3 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + casein substrate of TSSK3 Mus musculus ADP + phosphorylated casein
-
 ?
2.7.11.1 ATP + histone H1 substrate of TSSK3 Mus musculus ADP + phosphorylated histone H1
-
 ?
2.7.11.1 ATP + myelin basic protein substrate of TSSK3 Mus musculus ADP + phosphorylated myelin basic protein
-
 ?
2.7.11.1 ATP + RRSSSY peptide substrate of TSSK3 consisting of the consensus sequence Mus musculus ADP + RRSS-phosphoS-Y
-
 ?
2.7.11.1 ATP + testis-specific serine-threonine kinase 3 substrate of phosphoinositide-dependent kinase-1 leading to activation Mus musculus ADP + phosphorylated testis-specific serine-threonine kinase 3
-
 ?
2.7.11.1 ATP + testis-specific serine-threonine kinase 3 substrate of recombinant Myc-tagged catalytic subunit of PDK1 leading to activation Mus musculus ADP + phosphorylated testis-specific serine-threonine kinase 3
-
 ?
2.7.11.1 additional information TSSK3 performs autophosphorylation Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.1 More TSSK3 contains a T-loop structure Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 More the enzyme TSSK3 belongs to the family of testis-specific serine-threonine kinases Mus musculus
2.7.11.1 PDK1
-
 Mus musculus
2.7.11.1 phosphoinositide-dependent kinase-1
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 30
-
 TSSK3, and phosphoinositide-dependent kinase-1 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 7.4
-
 TSSK3 Mus musculus
2.7.11.1 7.5
-
 PDK1 assay at Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.1 6.8 8.2 TSSK3 shows a broad range pH-optimum Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.1 ATP dependent on, cannot be substituted by other nucleotide triphosphates like CTP or GTP Mus musculus