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Literature summary extracted from

  • Van Boxstael, S.; Maes, D.; Cunin, R.
    Aspartate transcarbamylase from the hyperthermophilic archaeon Pyrococcus abyssi. Insights into cooperative and allosteric mechanisms (2005), Febs J., 272, 2670-2683.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.3.2 ATP allosteric activator, up to 35% increase in Vmax Pyrococcus abyssi
2.1.3.2 additional information kinetic analysis of properties of allosteric effectors alone and in combination with each other Pyrococcus abyssi

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.2 CTP allosteric inhibitor Pyrococcus abyssi
2.1.3.2 additional information kinetic analysis of properties of allosteric effectors alone and in combination with each other Pyrococcus abyssi
2.1.3.2 UTP allosteric inhibitor Pyrococcus abyssi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.2 0.005
-
 Carbamoyl phosphate 37°C Pyrococcus abyssi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 17000
-
 6 * 35000 + 6 * 17000, 2C3/3R2 holoenzyme, calculated Pyrococcus abyssi
2.1.3.2 35000
-
 6 * 35000 + 6 * 17000, 2C3/3R2 holoenzyme, calculated Pyrococcus abyssi
2.1.3.2 301000
-
 gel filtration Pyrococcus abyssi

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Pyrococcus abyssi
-
 recombinant protein
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate cooperative mechanism of substrate binding Pyrococcus abyssi
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Pyrococcus abyssi phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 dodecamer 6 * 35000 + 6 * 17000, 2C3/3R2 holoenzyme, calculated Pyrococcus abyssi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.2 90
-
 half-life 260 min Pyrococcus abyssi
2.1.3.2 94
-
 half-life 190 min Pyrococcus abyssi
2.1.3.2 98
-
 half-life 100 min Pyrococcus abyssi