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Literature summary extracted from

  • Solaroli, N.; Bjerke, M.; Amiri, M.H.; Johansson, M.; Karlsson, A.
    Active site mutants of Drosophila melanogaster multisubstrate deoxyribonucleoside kinase (2003), Eur. J. Biochem., 270, 2879-2884.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.145 expression of wild-type and mutant enzymes in Escherichia coli as GST-fusion proteins Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.145 F114A site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme Drosophila melanogaster
2.7.1.145 F114Y site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme Drosophila melanogaster
2.7.1.145 I29H site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme Drosophila melanogaster
2.7.1.145 I29H/F114Y site-directed mutagenesis, active site mutant, inactive mutant Drosophila melanogaster
2.7.1.145 N28P site-directed mutagenesis, active site mutant, inactive mutant Drosophila melanogaster
2.7.1.145 N28P/I29H site-directed mutagenesis, active site mutant, inactive mutant Drosophila melanogaster
2.7.1.145 N28P/I29H/F114Y site-directed mutagenesis, active site mutant, inactive mutant Drosophila melanogaster
2.7.1.145 Q81D site-directed mutagenesis, active site mutant, inactive mutant Drosophila melanogaster
2.7.1.145 Q81N site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.145 0.0025
-
 2'-deoxythymidine pH 7.6, 37°C, recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 0.0031
-
 2'-deoxythymidine pH 7.6, 37°C, recombinant mutant F114A Drosophila melanogaster
2.7.1.145 0.0047
-
 2'-deoxythymidine pH 7.6, 37°C, recombinant mutant Q81N Drosophila melanogaster
2.7.1.145 0.022
-
 2'-deoxythymidine pH 7.6, 37°C, recombinant mutant F114Y Drosophila melanogaster
2.7.1.145 0.1
-
 2'-deoxythymidine above, pH 7.6, 37°C, recombinant mutants I29H and Q81D Drosophila melanogaster
2.7.1.145 0.373
-
 2'-deoxyadenosine pH 7.6, 37°C, recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 0.625
-
 2'-deoxyadenosine pH 7.6, 37°C, recombinant mutant Q81N Drosophila melanogaster
2.7.1.145 0.985
-
 2'-deoxyguanosine pH 7.6, 37°C, recombinant mutant Q81N Drosophila melanogaster
2.7.1.145 2
-
 2'-deoxyguanosine pH 7.6, 37°C, recombinant wild-type enzyme Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.145 recombinant wild-type and mutant GST-fusion enzymes from Escherichia coli by glutathione affinity chromatography Drosophila melanogaster

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.145 additional information
-
 substrate specificities of wild-type and mutant enzymes Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.145 ATP + 1-beta-D-arabinofuranosylcytosine
-
 Drosophila melanogaster ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2',2'-difluorodeoxycytidine
-
 Drosophila melanogaster ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyadenosine
-
 Drosophila melanogaster ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine
-
 Drosophila melanogaster ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine poor substrate for the wild-type enzyme Drosophila melanogaster ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine
-
 Drosophila melanogaster ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2-chlorodeoxyadenosine
-
 Drosophila melanogaster ADP + 2-chlorodeoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + E-5-(2-bromovinyl)-2'-deoxyuridine
-
 Drosophila melanogaster ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate
-
 ?
2.7.1.145 additional information substrate specificities of wild-type and mutant enzymes, overview, no or poor activity with ganciclovir Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.145 dNK
-
 Drosophila melanogaster
2.7.1.145 multisubstrate deoxyribonucleoside kinase
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.145 37
-
 assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.145 7.6
-
 assay at Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.145 ATP
-
 Drosophila melanogaster