Data extracted from this reference:
Cloned(Commentary)
2.7.1.145
expression of wild-type and mutant enzymes in Escherichia coli as GST-fusion proteins
Drosophila melanogaster
Engineering
2.7.1.145
F114A
site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
F114Y
site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
I29H
site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
I29H/F114Y
site-directed mutagenesis, active site mutant, inactive mutant
Drosophila melanogaster
2.7.1.145
N28P
site-directed mutagenesis, active site mutant, inactive mutant
Drosophila melanogaster
2.7.1.145
N28P/I29H
site-directed mutagenesis, active site mutant, inactive mutant
Drosophila melanogaster
2.7.1.145
N28P/I29H/F114Y
site-directed mutagenesis, active site mutant, inactive mutant
Drosophila melanogaster
2.7.1.145
Q81D
site-directed mutagenesis, active site mutant, inactive mutant
Drosophila melanogaster
2.7.1.145
Q81N
site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme
Drosophila melanogaster
KM Value [mM]
2.7.1.145
0.0025
2'-deoxythymidine
pH 7.6, 37°C, recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
0.0031
2'-deoxythymidine
pH 7.6, 37°C, recombinant mutant F114A
Drosophila melanogaster
2.7.1.145
0.0047
2'-deoxythymidine
pH 7.6, 37°C, recombinant mutant Q81N
Drosophila melanogaster
2.7.1.145
0.022
2'-deoxythymidine
pH 7.6, 37°C, recombinant mutant F114Y
Drosophila melanogaster
2.7.1.145
0.1
2'-deoxythymidine
above, pH 7.6, 37°C, recombinant mutants I29H and Q81D
Drosophila melanogaster
2.7.1.145
0.373
2'-deoxyadenosine
pH 7.6, 37°C, recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
0.625
2'-deoxyadenosine
pH 7.6, 37°C, recombinant mutant Q81N
Drosophila melanogaster
2.7.1.145
0.985
2'-deoxyguanosine
pH 7.6, 37°C, recombinant mutant Q81N
Drosophila melanogaster
2.7.1.145
2
2'-deoxyguanosine
pH 7.6, 37°C, recombinant wild-type enzyme
Drosophila melanogaster
Organism
2.7.1.145
Drosophila melanogaster
Purification (Commentary)
2.7.1.145
recombinant wild-type and mutant GST-fusion enzymes from Escherichia coli by glutathione affinity chromatography
Drosophila melanogaster
Specific Activity [micromol/min/mg]
2.7.1.145
additional information
substrate specificities of wild-type and mutant enzymes
Drosophila melanogaster
Substrates and Products (Substrate)
2.7.1.145
ATP + 1-beta-D-arabinofuranosylcytosine
661629
Drosophila melanogaster
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
?
2.7.1.145
ATP + 2',2'-difluorodeoxycytidine
661629
Drosophila melanogaster
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
?
2.7.1.145
ATP + 2'-deoxyadenosine
661629
Drosophila melanogaster
ADP + 2'-deoxyadenosine 5'-phosphate
?
2.7.1.145
ATP + 2'-deoxycytidine
661629
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
?
2.7.1.145
ATP + 2'-deoxyguanosine
poor substrate for the wild-type enzyme
661629
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
?
2.7.1.145
ATP + 2'-deoxythymidine
661629
Drosophila melanogaster
ADP + 2'-deoxythymidine 5'-phosphate
?
2.7.1.145
ATP + 2-chlorodeoxyadenosine
661629
Drosophila melanogaster
ADP + 2-chlorodeoxyadenosine 5'-phosphate
?
2.7.1.145
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine
661629
Drosophila melanogaster
ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate
?
2.7.1.145
additional information
substrate specificities of wild-type and mutant enzymes, overview, no or poor activity with ganciclovir
661629
Drosophila melanogaster
?
Synonyms
2.7.1.145
dNK
Drosophila melanogaster
2.7.1.145
multisubstrate deoxyribonucleoside kinase
Drosophila melanogaster
Temperature Optimum [°C]
2.7.1.145
37
assay at
Drosophila melanogaster
pH Optimum
2.7.1.145
7.6
assay at
Drosophila melanogaster
Cofactor
2.7.1.145
ATP
Drosophila melanogaster
Cloned(Commentary) (protein specific)
2.7.1.145
expression of wild-type and mutant enzymes in Escherichia coli as GST-fusion proteins
Drosophila melanogaster
Cofactor (protein specific)
2.7.1.145
ATP
Drosophila melanogaster
Engineering (protein specific)
2.7.1.145
F114A
site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
F114Y
site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
I29H
site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme
Drosophila melanogaster
2.7.1.145
I29H/F114Y
site-directed mutagenesis, active site mutant, inactive mutant
Drosophila melanogaster
2.7.1.145
N28P
site-directed mutagenesis, active site mutant, inactive mutant
Drosophila melanogaster
2.7.1.145
N28P/I29H
site-directed mutagenesis, active site mutant, inactive mutant
Drosophila melanogaster
2.7.1.145
N28P/I29H/F114Y
site-directed mutagenesis, active site mutant, inactive mutant
Drosophila melanogaster
2.7.1.145
Q81D
site-directed mutagenesis, active site mutant, inactive mutant
Drosophila melanogaster
2.7.1.145
Q81N
site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme
Drosophila melanogaster
KM Value [mM] (protein specific)
2.7.1.145
0.0025
2'-deoxythymidine
pH 7.6, 37°C, recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
0.0031
2'-deoxythymidine
pH 7.6, 37°C, recombinant mutant F114A
Drosophila melanogaster
2.7.1.145
0.0047
2'-deoxythymidine
pH 7.6, 37°C, recombinant mutant Q81N
Drosophila melanogaster
2.7.1.145
0.022
2'-deoxythymidine
pH 7.6, 37°C, recombinant mutant F114Y
Drosophila melanogaster
2.7.1.145
0.1
2'-deoxythymidine
above, pH 7.6, 37°C, recombinant mutants I29H and Q81D
Drosophila melanogaster
2.7.1.145
0.373
2'-deoxyadenosine
pH 7.6, 37°C, recombinant wild-type enzyme
Drosophila melanogaster
2.7.1.145
0.625
2'-deoxyadenosine
pH 7.6, 37°C, recombinant mutant Q81N
Drosophila melanogaster
2.7.1.145
0.985
2'-deoxyguanosine
pH 7.6, 37°C, recombinant mutant Q81N
Drosophila melanogaster
2.7.1.145
2
2'-deoxyguanosine
pH 7.6, 37°C, recombinant wild-type enzyme
Drosophila melanogaster
Purification (Commentary) (protein specific)
2.7.1.145
recombinant wild-type and mutant GST-fusion enzymes from Escherichia coli by glutathione affinity chromatography
Drosophila melanogaster
Specific Activity [micromol/min/mg] (protein specific)
2.7.1.145
additional information
substrate specificities of wild-type and mutant enzymes
Drosophila melanogaster
Substrates and Products (Substrate) (protein specific)
2.7.1.145
ATP + 1-beta-D-arabinofuranosylcytosine
661629
Drosophila melanogaster
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
?
2.7.1.145
ATP + 2',2'-difluorodeoxycytidine
661629
Drosophila melanogaster
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
?
2.7.1.145
ATP + 2'-deoxyadenosine
661629
Drosophila melanogaster
ADP + 2'-deoxyadenosine 5'-phosphate
?
2.7.1.145
ATP + 2'-deoxycytidine
661629
Drosophila melanogaster
ADP + 2'-deoxycytidine 5'-phosphate
?
2.7.1.145
ATP + 2'-deoxyguanosine
poor substrate for the wild-type enzyme
661629
Drosophila melanogaster
ADP + 2'-deoxyguanosine 5'-phosphate
?
2.7.1.145
ATP + 2'-deoxythymidine
661629
Drosophila melanogaster
ADP + 2'-deoxythymidine 5'-phosphate
?
2.7.1.145
ATP + 2-chlorodeoxyadenosine
661629
Drosophila melanogaster
ADP + 2-chlorodeoxyadenosine 5'-phosphate
?
2.7.1.145
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine
661629
Drosophila melanogaster
ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate
?
2.7.1.145
additional information
substrate specificities of wild-type and mutant enzymes, overview, no or poor activity with ganciclovir
661629
Drosophila melanogaster
?
Temperature Optimum [°C] (protein specific)
2.7.1.145
37
assay at
Drosophila melanogaster
pH Optimum (protein specific)
2.7.1.145
7.6
assay at
Drosophila melanogaster