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Literature summary extracted from

  • He, B.; Weber, G.F.
    Phosphorylation of NF-kappaB proteins by cyclic GMP-dependent kinase. A noncanonical pathway to NF-kappaB activation (2003), Eur. J. Biochem., 270, 2174-2185.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.12 DNA sequence determination of isozyme PKGIalpha, functional co-expression of His-tagged enzyme in 293T cells with human NF-kappaB p49 and p50, NF-kappaB activates PKG, which increases 5fold transactivation activity of p65 from consensus sites but not from a nonconsensus NF-kappaB reporter Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.12 Rp-8-pCPT-cGMPS
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.12 Mg2+
-
 Mus musculus
2.7.11.12 Mg2+
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.12 Bos taurus
-
 isozyme PKGIalpha
-
2.7.11.12 Mus musculus
-
 BALB/c mice, isozyme PKGIalpha
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.12 commercial preparation native purified from lung or recombinant Bos taurus
-
2.7.11.12 kidney
-
 Mus musculus
-
2.7.11.12 lung
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.12 ATP + EKRKRTYETF p65-derived synthetic peptide substrate Mus musculus ADP + phosphorylated EKRKRTYETF
-
 ?
2.7.11.12 ATP + Kemptide i.e. LRRASLG Mus musculus ADP + phosphorylated Kemptide i.e. LRRA-phosphoserine-LG ?
2.7.11.12 ATP + Kemptide i.e. LRRASLG Bos taurus ADP + phosphorylated Kemptide i.e. LRRA-phosphoserine-LG ?
2.7.11.12 ATP + MQLRRPSDRE p65-derived synthetic peptide substrate Mus musculus ADP + phosphorylated MQLRRPSDRE
-
 ?
2.7.11.12 ATP + NF-kappaB recombinant human NF-kappaB p49 and p50 expressed in 293T cells Mus musculus ADP + phosphorylated NF-kappaB
-
 ?
2.7.11.12 ATP + NF-kappaB recombinant human NF-kappaB p49 and p50 expressed in 293T cells Bos taurus ADP + phosphorylated NF-kappaB
-
 ?
2.7.11.12 ATP + p65 activity with recombinant wild-type p65 and mutants T305A and S276A Mus musculus ADP + phosphorylated p65
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.12 cyclic GMP-dependent kinase
-
 Mus musculus
2.7.11.12 cyclic GMP-dependent kinase
-
 Bos taurus
2.7.11.12 PKG
-
 Mus musculus
2.7.11.12 PKG
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.12 22
-
 assay at room temperature Mus musculus
2.7.11.12 22
-
 assay at room temperature Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.12 6.9
-
 assay at, substrate Kemptide Mus musculus
2.7.11.12 6.9
-
 assay at, substrate Kemptide Bos taurus
2.7.11.12 7.5
-
 assay at, substrate NF-kappaB Mus musculus
2.7.11.12 7.5
-
 assay at, substrate NF-kappaB Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.12 ATP
-
 Mus musculus
2.7.11.12 ATP
-
 Bos taurus