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Literature summary extracted from

  • Eggink, L.L.; LoBrutto, R.; Brune, D.C.; Brusslan, J.; Yamasato, A.; Tanaka, A.; Hoober, J.K.
    Synthesis of chlorophyll b: localization of chlorophyllide a oxygenase and discovery of a stable radical in the catalytic subunit (2004), BMC Plant Biol., 4, 5.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.122 expression in Escherichia coli in membranes, phylogenetic tree Arabidopsis thaliana
1.14.13.122 expression of GFP-tagged enzyme in Chlamydomonas reinhardtii cells, phylogenetic tree Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.122 chloroplast envelope inner membrane Arabidopsis thaliana 9507
-
1.14.13.122 chloroplast envelope inner membrane, in light and dark grown cells Chlamydomonas reinhardtii 9507
-
1.14.13.122 thylakoid membrane
-
 Arabidopsis thaliana 42651
-
1.14.13.122 thylakoid membrane only in light grown cells Chlamydomonas reinhardtii 42651
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.122 Fe2+ the enzyme contains a [2Fe-2S] Rieske cluster and a mononuclear non-hem iron-binding site Chlamydomonas reinhardtii
1.14.13.122 Fe2+ the enzyme contains a [2Fe-2S] Rieske cluster and a mononuclear non-hem iron-binding site Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.122 54000
-
 x * 54000, recombinant enzyme, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.122 7-hydroxychlorophyllide a + O2 + NADPH Chlamydomonas reinhardtii
-
 chlorophyllide b + H2O + NADP+
-
 ?
1.14.13.122 7-hydroxychlorophyllide a + O2 + NADPH Arabidopsis thaliana
-
 chlorophyllide b + H2O + NADP+
-
 ?
1.14.13.122 7-hydroxychlorophyllide a + O2 + NADPH Chlamydomonas reinhardtii cw15
-
 chlorophyllide b + H2O + NADP+
-
 ?
1.14.13.122 chlorophyllide a + O2 + NADPH + H+ Chlamydomonas reinhardtii
-
 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
1.14.13.122 chlorophyllide a + O2 + NADPH + H+ Arabidopsis thaliana
-
 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
1.14.13.122 chlorophyllide a + O2 + NADPH + H+ Chlamydomonas reinhardtii cw15
-
 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
1.14.13.122 additional information Chlamydomonas reinhardtii chlorophyll b is required for assembly of the light-harvesting complexes ?
-
 ?
1.14.13.122 additional information Arabidopsis thaliana chlorophyll b is required for assembly of the light-harvesting complexes ?
-
 ?
1.14.13.122 additional information Chlamydomonas reinhardtii cw15 chlorophyll b is required for assembly of the light-harvesting complexes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.122 Arabidopsis thaliana
-
-
-
1.14.13.122 Chlamydomonas reinhardtii
-
-
-
1.14.13.122 Chlamydomonas reinhardtii cw15
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.122 chlorophyllide a + 2 O2 + 2 NADPH + 2 H+ = chlorophyllide b + 3 H2O + 2 NADP+ reaction mechanism, the catalytic site harbors a stable radical formed by Tyr422 which is involved in the catalytic reaction Chlamydomonas reinhardtii
a
1.14.13.122 chlorophyllide a + 2 O2 + 2 NADPH + 2 H+ = chlorophyllide b + 3 H2O + 2 NADP+ reaction mechanism, the catalytic site harbors a stable radical formed by Tyr518 which is involved in the catalytic reaction Arabidopsis thaliana
a
1.14.13.122 chlorophyllide a + O2 + NADPH + H+ = 71-hydroxychlorophyllide a + H2O + NADP+ reaction mechanism, the catalytic site harbors a stable radical formed by Tyr422 which is involved in the catalytic reaction Chlamydomonas reinhardtii
a
1.14.13.122 chlorophyllide a + O2 + NADPH + H+ = 71-hydroxychlorophyllide a + H2O + NADP+ reaction mechanism, the catalytic site harbors a stable radical formed by Tyr518 which is involved in the catalytic reaction Arabidopsis thaliana
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.122 leaf primary Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.122 7-hydroxychlorophyllide a + O2 + NADPH
-
 Chlamydomonas reinhardtii chlorophyllide b + H2O + NADP+
-
 ?
1.14.13.122 7-hydroxychlorophyllide a + O2 + NADPH
-
 Arabidopsis thaliana chlorophyllide b + H2O + NADP+
-
 ?
1.14.13.122 7-hydroxychlorophyllide a + O2 + NADPH
-
 Chlamydomonas reinhardtii cw15 chlorophyllide b + H2O + NADP+
-
 ?
1.14.13.122 chlorophyllide a + O2 + NADPH + H+
-
 Chlamydomonas reinhardtii 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
1.14.13.122 chlorophyllide a + O2 + NADPH + H+
-
 Arabidopsis thaliana 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
1.14.13.122 chlorophyllide a + O2 + NADPH + H+
-
 Chlamydomonas reinhardtii cw15 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
1.14.13.122 additional information chlorophyll b is required for assembly of the light-harvesting complexes Chlamydomonas reinhardtii ?
-
 ?
1.14.13.122 additional information chlorophyll b is required for assembly of the light-harvesting complexes Arabidopsis thaliana ?
-
 ?
1.14.13.122 additional information chlorophyll b is required for assembly of the light-harvesting complexes Chlamydomonas reinhardtii cw15 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.122 ? x * 54000, recombinant enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.14.13.122 CAO
-
 Chlamydomonas reinhardtii
1.14.13.122 CAO
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.122 NADPH
-
 Chlamydomonas reinhardtii
1.14.13.122 NADPH
-
 Arabidopsis thaliana