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Literature summary extracted from

  • Yamada, N.; Motoyama, T.; Nakasako, M.; Kagabu, S.; Kudo, T.; Yamaguchi, I.
    Enzymatic characterization of scytalone dehydratase Val75Met variant found in melanin biosynthesis dehydratase inhibitor (MBI-D) resistant strains of the rice blast fungus (2004), Biosci. Biotechnol. Biochem., 68, 615-621.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.94 overexpression of V75M in Escherichia coli with or without an N-terminal histidine-tag Pyricularia oryzae

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.94 V75M inhibition of the mutant enzyme by (1RS,3RS)-2,2-dichloro-N-[(R)-1-(4-chlorophenyl)ethyl]-1-ethyl-3-methylcyclopropanecarboxamide is more than 200fold reduced in comparison with that of the wild-type, about 2fold reduced turnover number for scytalone as compared to wild-type enzyme Pyricularia oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.94 (1RS,3RS)-2,2-dichloro-N-[(R)-1-(4-chlorophenyl)ethyl]-1-ethyl-3-methylcyclopropanecarboxamide i.e. carpropamid Pyricularia oryzae
4.2.1.94 KTU3615A competitive Pyricularia oryzae
4.2.1.94 KTU3615B competitive Pyricularia oryzae
4.2.1.94 KTU3616A competitive Pyricularia oryzae
4.2.1.94 KTU3616B competitive Pyricularia oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.94 0.021
-
 Scytalone mutant enzyme V75M with His-tag Pyricularia oryzae
4.2.1.94 0.022
-
 Scytalone wild-type enzyme Pyricularia oryzae
4.2.1.94 0.023
-
 Scytalone mutant enzyme V75M without His-tag Pyricularia oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.94 21000
-
 x * 21000, calculation from nucleotide sequence Pyricularia oryzae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.94 Pyricularia oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.94
-
 Pyricularia oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.94 scytalone
-
 Pyricularia oryzae 1,3,8-trihydroxynaphthalene + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.94 ? x * 21000, calculation from nucleotide sequence Pyricularia oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.94 3 6 Scytalone mutant enzyme V75M with His-tag Pyricularia oryzae
4.2.1.94 41
-
 Scytalone mutant enzyme V75M without His-tag Pyricularia oryzae
4.2.1.94 70
-
 Scytalone wild-type enzyme Pyricularia oryzae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.94 0.0000001
-
 KTU3616B pH 7.0, 30°C, wild-type enzme Pyricularia oryzae
4.2.1.94 0.000011
-
 KTU3616A pH 7.0, 30°C, wild-type enzme Pyricularia oryzae
4.2.1.94 0.000024
-
 KTU3615A pH 7.0, 30°C, wild-type enzme Pyricularia oryzae